Enhancement of polyunsaturated fatty acid production by Tn5 transposon in Shewanella baltica

被引：26

作者：

Amiri-Jami, Mitra

Wang, Haifeng

Kakuda, Yukio

Griffiths, Mansel W. ^{[1
]}

机构：

[1] Univ Guelph, Canadian Res Inst Food Safety, Guelph, ON N1G 2W1, Canada

[2] Univ Guelph, Dept Food Sci, Guelph, ON N1G 2W1, Canada

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 15期

基金：

加拿大自然科学与工程研究理事会;

关键词：

docosahexaenoic acid; eicosapentaenoic acid; Shewanella; transposon mutagenesis;

D O I：

10.1007/s10529-006-9077-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transposon Tn5 mutagenesis was used to generate random mutations in Shewanella baltica MAC1, a polyunsaturated fatty acid (PUFA)-producing bacterium. Three mutants produced 3-5 times more eicosapentaenoic acid (EPA 20:5 n-3) compared to the wild type at 10 degrees C. One of the mutants produced 0.3 mg EPA g(-1) stop when grown at high temperature (30 degrees C). Moreover, 2 mg docosahexaenoic acid (DHA 22:6 n-3) g(-1) was produced by S. baltica mutants at 4 degrees C. Sequencing of insertion mutation(s) showed 96% homology to trimethylamine N-oxide (TMAO) reductase gene and 85% homology to rRNA operons of E. coli. Tn5 transposon mutagenesis therefore is a suitable technique to increase PUFA formation in bacteria.

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收藏

页码：1187 / 1192

页数：6

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