A highly efficient 24-condition matrix for the crystallization of nucleic acid fragments

被引：69

作者：

Berger, I

Kang, CH

Sinha, N

Wolters, M

Rich, A

机构：

[1] HANNOVER MED SCH, DEPT BIOPHYS CHEM, D-30623 HANNOVER, GERMANY

[2] WASHINGTON STATE UNIV, DEPT BIOCHEM & BIOPHYS, PULLMAN, WA 99164 USA

[3] MILLIPORE CORP, BEDFORD, MA 01730 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1996年 / 52卷

关键词：

D O I：

10.1107/S0907444995013564

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A matrix screen Is presented with optimized conditions for the crystallization of nucleic acid fragments. The screen consists of 24 conditions only and utilizes minimal amounts of sample. It has been tested on a series of DNA and RNA oligomers, and yielded diffraction-quality crystals of many specimens including drug-DNA complexes. Preliminary X-ray analysis of the crystals is reported.

引用

收藏

页码：465 / 468

页数：4

相关论文

共 21 条

[1] EXTENSION OF THE 4-STRANDED INTERCALATED CYTOSINE MOTIF BY ADENINE-CENTER-DOT-ADENINE BASE-PAIRING IN THE CRYSTAL-STRUCTURE OF D(CCCAAT) [J].

BERGER, I ;

KANG, C ;

FREDIAN, A ;

RATLIFF, R ;

MOYZIS, R ;

RICH, A .

NATURE STRUCTURAL BIOLOGY, 1995, 2 (05) :416-429

[2] MOLECULAR-STRUCTURE OF THE HALOGENATED ANTICANCER DRUG IODODOXORUBICIN COMPLEXED WITH D(TGTACA) AND D(CGATCG) [J].

BERGER, I ;

SU, L ;

SPITZNER, JR ;

KANG, C ;

BURKE, TG ;

RICH, A .

NUCLEIC ACIDS RESEARCH, 1995, 23 (21) :4488-4494

[3] CRYSTAL-STRUCTURE OF A 4-STRANDED INTERCALATED DNA - D(C-4) [J].

CHEN, LQ ;

CAI, L ;

ZHANG, XH ;

RICH, A .

BIOCHEMISTRY, 1994, 33 (46) :13540-13546

[4] THE ANATOMY OF A-DNA, B-DNA, AND Z-DNA [J].

DICKERSON, RE ;

DREW, HR ;

CONNER, BN ;

WING, RM ;

FRATINI, AV ;

KOPKA, ML .

SCIENCE, 1982, 216 (4545) :475-485

[5] HELIX GEOMETRY AND HYDRATION IN A-DNA, B-DNA, AND Z-DNA [J].

DICKERSON, RE ;

DREW, HR ;

CONNER, BN ;

KOPKA, ML ;

PJURA, PE .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1982, 47 :13-24

[6]

DICKERSON RE, 1991, HUM GENET, V87, P377

[7]

DOCKBREGEON AC, 1991, CRYSTALLIZATION NUCL, P145

[8] CRYSTALLIZATION OF RIBOZYMES AND SMALL RNA MOTIFS BY A SPARSE-MATRIX APPROACH [J].

DOUDNA, JA ;

GROSSHANS, C ;

GOODING, A ;

KUNDROT, CE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (16) :7829-7833

[9] A TETRAMERIC DNA-STRUCTURE WITH PROTONATED CYTOSINE.CYTOSINE BASE-PAIRS [J].

GEHRING, K ;

LEROY, JL ;

GUERON, M .

NATURE, 1993, 363 (6429) :561-565

[10] SPARSE-MATRIX SAMPLING - A SCREENING METHOD FOR CRYSTALLIZATION OF PROTEINS [J].

JANCARIK, J ;

KIM, SH .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 :409-411