Crystallization of membrane proteins

被引：180

作者：

Ostermeier, C ^{[1
]}

Michel, H ^{[1
]}

机构：

[1] MAX PLANCK INST BIOPHYS,ABT MOL MEMBRANBIOL,D-60528 FRANKFURT,GERMANY

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1997年 / 7卷 / 05期

关键词：

D O I：

10.1016/S0959-440X(97)80080-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five new membrane protein structures have been determined since 1995 using X-ray crystallography: bacterial light-harvesting complex; bacterial and mitochondrial cytochrome c oxidases; mitochondrial bc(1) complex; and a-hemolysin. These successes are partly based on advances in the crystallization procedures for integral membrane proteins. Variation of the size of the detergent micelle and/or increasing the size of the polar surface of the membrane protein is the most important route to well-ordered membrane protein crystals. The use of bicontinuous lipidic cubic phases also appears to be promising. (C) Current Biology Ltd ISSN 0959-440X

引用

页码：697 / 701

页数：5

共 25 条

[1] Strategies for crystallizing membrane proteins [J].

Garavito, RM ;

Picot, D ;

Loll, PJ .

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1996, 28 (01) :13-27

[2] THE GROWTH AND CHARACTERIZATION OF MEMBRANE-PROTEIN CRYSTALS [J].

GARAVITO, RM ;

MARKOVICHOUSLEY, Z ;

JENKINS, JA .

JOURNAL OF CRYSTAL GROWTH, 1986, 76 (03) :701-709

[3] DETERMINATION OF THE NUMBER OF DETERGENT MOLECULES ASSOCIATED WITH THE REACTION-CENTER PROTEIN ISOLATED FROM THE PHOTOSYNTHETIC BACTERIUM RHODOPSEUDOMONAS-VIRIDIS - EFFECTS OF THE AMPHIPHILIC MOLECULE 1,2,3-HEPTANETRIOL [J].

GAST, P ;

HEMELRIJK, P ;

HOFF, AJ .

FEBS LETTERS, 1994, 337 (01) :39-42

[4]

GRIESSHAMMER R, 1995, Q REV BIOPHYS, V28, P315

[5] STRUCTURE AT 2.8-ANGSTROM RESOLUTION OF CYTOCHROME-C-OXIDASE FROM PARACOCCUS-DENITRIFICANS [J].

IWATA, S ;

OSTERMEIER, C ;

LUDWIG, B ;

MICHEL, H .

NATURE, 1995, 376 (6542) :660-669

[6] ENGINEERED FV FRAGMENTS AS A TOOL FOR THE ONE-STEP PURIFICATION OF INTEGRAL MULTISUBUNIT MEMBRANE-PROTEIN COMPLEXES [J].

KLEYMANN, G ;

OSTERMEIER, C ;

LUDWIG, B ;

SKERRA, A ;

MICHEL, H .

BIO-TECHNOLOGY, 1995, 13 (02) :155-160

[7] The crystal structure of the light-harvesting complex II (B800-850) from Rhodospirillum molischianum [J].

Koepke, J ;

Hu, XC ;

Muenke, C ;

Schulten, K ;

Michel, H .

STRUCTURE, 1996, 4 (05) :581-597

[8] 3-DIMENSIONAL CRYSTALLIZATION OF MEMBRANE-PROTEINS [J].

KUHLBRANDT, W .

QUARTERLY REVIEWS OF BIOPHYSICS, 1988, 21 (04) :429-&

[9] Lipidic cubic phases: A novel concept for the crystallization of membrane proteins [J].

Landau, EM ;

Rosenbusch, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14532-14535

[10] PRELIMINARY CRYOCRYSTALLOGRAPHIC STUDY OF THE MITOCHONDRIAL CYTOCHROME BC(1) COMPLEX - IMPROVED CRYSTALLIZATION AND FLASH-COOLING OF A LARGE MEMBRANE-PROTEIN [J].

LEE, JW ;

CHAN, M ;

LAW, TV ;

KWON, HJ ;

JAP, BK .

JOURNAL OF MOLECULAR BIOLOGY, 1995, 252 (01) :15-19

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