A column design for reducing viscous fingering in size exclusion chromatography

被引：28

作者：

Fernandez, EJ

Norton, TT

Jung, WC

Tsavalas, JG

机构：

[1] Department of Chemical Engineering, University of Virginia, Charlottesville

[2] Ashburn, 22011

来源：

BIOTECHNOLOGY PROGRESS | 1996年 / 12卷 / 04期

关键词：

D O I：

10.1021/bp960031r

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Under high concentration overload conditions, resolution during size exclusion chromatography of proteins can be compromised due to nonuniform flow caused by the viscous fingering flow instability. In this work, the nonuniform flow under these chromatographic conditions is analyzed by numerical simulation and magnetic resonance imaging. On the basis of an improved understanding of the flow phenomena, a new column design is proposed. The effectiveness of the design is evaluated by chromatography, magnetic resonance imaging, and numerical simulation.

引用

页码：480 / 487

页数：8

共 30 条

[1]

ARAKTINGI UG, 1990, SPE ANN TECHN M TULS

[2] VISUALIZATION OF CHROMATOGRAPHIC SEPARATIONS BY NMR IMAGING [J].