First cleavage of the mouse embryo responds to change in egg shape at fertilization

被引:74
作者
Gray, D
Plusa, B
Piotrowska, K
Na, J
Tom, B
Glover, DM
Zernicka-Goetz, M
机构
[1] Gurdon Inst, Wellcome Trust Canc Res UK, Cambridge CB2 1QR, England
[2] Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England
[3] Inst Publ Hlth, MRC, Biostat Unit, Cambridge CB2 2SR, England
基金
英国惠康基金;
关键词
D O I
10.1016/j.cub.2004.02.031
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although mouse development is regulative, the cleavage pattern of the embryo is not random [1, 2]. The first cleavage tends to relate to the site of the previous meiosis [3, 4]. Sperm entry might provide a second cue, but evidence for [5, 6] and against [7, 8] this is indirect and has been debated [9, 10]. To resolve whether sperm entry position relates to the first cleavage, we have followed development from fertilization by time-lapse imaging. This directly showed cytokinesis passes close to the site of the previous meiosis and to both the sperm entry site and trajectory of the male pronucleus in a significant majority of eggs. We detected asymmetric distribution of Par6 protein in relation to the site of meiosis, but not sperm entry. Unexpectedly, we found the egg becomes flattened upon fertilization in an actin-mediated process. The sperm entry position tends to lie at one end of the short axis along which cleavage will pass. When we manipulated eggs to change their shape, this repositioned the cleavage plane such that eggs divided along their experimentally imposed short axis. Such manipulated eggs were able to develop to term, emphasizing the regulative nature of their development.
引用
收藏
页码:397 / 405
页数:9
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