Genetic transformation of the mosquito pathogenic fungus Culicinomyces clavisporus

被引：5

作者：

Campbell, DI

Kinghorn, JR ^{[1
]}

Kana'n, GJM

Unkles, SE

Panter, C

机构：

[1] Univ St Andrews, Sch Biol, St Andrews KY16 9AJ, Fife, Scotland

[2] Monash Univ, Dept Microbiol, Clayton, Vic 3800, Australia

[3] Monash Univ, Sch Appl Sci, Churchill, Vic 3806, Australia

来源：

BIOCONTROL SCIENCE AND TECHNOLOGY | 2002年 / 12卷 / 03期

关键词：

fungal pathogen of mosquitoes; Culicinomyces clavisporus; genetic transformation; antibiotic resistance;

D O I：

10.1080/09583150220128176

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The mosquito fungal pathogen Culicinomyces clavisporus was found to be sensitive to the antibiotics hygromycin B and phleomycin but resistant to kanamycin and geneticin. Protoplasts were genetically transformed to give strains resistant to phleomycin and hygromycin B. The results show that multiple integrations of transforming DNA can occur and that heterologous fungal gene expression signals are functional in C. clavisporus.

引用

页码：395 / 399

页数：5

共 18 条

[1]

CLUTTERBUCK AJ, 1974, HDB GENETICS, V1, P47

[2] NEW FUNGUS WHICH ATTACKS MOSQUITOS AND RELATED DIPTERA [J].