Molecular cloning, expression and function of the murine CB2 peripheral cannabinoid receptor

被引：156

作者：

Shire, D ^{[1
]}

Calandra, B ^{[1
]}

RinaldiCarmona, M ^{[1
]}

Oustric, D ^{[1
]}

Pessegue, B ^{[1
]}

BonninCabanne, O ^{[1
]}

LeFur, G ^{[1
]}

Caput, D ^{[1
]}

Ferrara, P ^{[1
]}

机构：

[1] SANOFI RECH,F-34184 MONTPELLIER 04,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1307卷 / 02期

关键词：

cannabinoid receptor; peripheral; binding property; adenylyl cyclase inhibition; (mouse);

D O I：

10.1016/0167-4781(96)00047-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned the peripheral cannabinoid receptor, mCB2, from a mouse splenocyte cDNA library. The 3.7 kb sequence contains an open reading frame encoding a protein of 347 residues sharing 82% overall identity with the only other known peripheral receptor, human CB2 (hCB2) and shorter than hCB2 by 13 amino acids at the carboxyl terminus. Binding experiments with membranes from COS-3 cells transiently expressing mCB2 showed that the synthetic cannabinoid WIN 55212-2 had a 6-fold lower affinity for mCB2 than for hCB2, whereas both receptors showed similar affinities for the agonists CP 55,940, Delta(9)-THC and anandamide and almost no affinity for the central receptor-(CB1) specific antagonist SR 141716A. Both hCB2 and mCB2 mediate agonist-stimulated inhibition of forskolin-induced cAMP production in CHO cell lines permanently expressing the receptors SR 141716A failed to antagonize this activity in either cell line, confirming its specificity for CB1.

引用

页码：132 / 136

页数：5

共 14 条

[1] CANNABINOID-RECEPTOR EXPRESSION IN HUMAN-LEUKOCYTES [J].