TGF-β of lung cancer microenvironment upregulates B7H1 and GITRL expression in dendritic cells and is associated with regulatory T cell generation

被引：94

作者：

Ni, Xiao Yan ^{[1
]}

Sui, Hua Xiu ^{[1
]}

Liu, Yao ^{[1
]}

Ke, Shi Zhong ^{[1
]}

Wang, Yi Nan ^{[1
]}

Gao, Feng Guang ^{[1
,2
]}

机构：

[1] Xiamen Univ, Coll Med, Dept Immunol, Xiamen 361005, Peoples R China

[2] Jiao Tong Univ, Canc Inst Shanghai, State Key Lab Oncogenes & Related Genes, Shanghai 200030, Peoples R China

来源：

ONCOLOGY REPORTS | 2012年 / 28卷 / 02期

关键词：

regulatory T cells; dendritic cells; B7H1; GITRL; tumor microenvironment; TUMOR MICROENVIRONMENT; GROWTH; EXPANSION; STIMULATE; SYSTEM; STROMA;

D O I：

10.3892/or.2012.1822

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

The effects of TGF-beta on dendritic cells (DCs) on the tumor microenvironment are not well understood. We report, here, the establishment of an in vitro lung cancer microenvironment by co-incubation of seminaphtharho-dafluor (SNARE) labeled Lewis lung cancer (LLC) cells, carboxyfluorescein succinimidyl ester (CFSE) labeled fibroblasts and 4-chloromethyl-7-hydroxycoumarin (CM HC) labeled DCs. Raw 264.7, EL4 and NCI-H446 cells were able to synthesize TGF-beta which was determined by flow cytometry and western blotting, respectively. Furthermore, TGF-beta efficiently increased regulatory T-cell (Treg) expansion and upregulated DC B7H1 and GITRL expression. TGF-beta and the co-incubation of LLC cells, fibroblasts with DCs could augment the expression of B7H1 and GITRL molecules of DCs. The data presented here indicate that the B7H1 and GITRL molecules may play an important role in TGF-beta-induced Treg expansion of lung cancer microenvironment.

引用

页码：615 / 621

页数：7

共 34 条

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