Effects of chemical modifications and immobilization of glucose oxidase onto acrylonitrile copolymer membranes on membrane potential and membrane charge density

被引:10
作者
Godjevargova, T [1 ]
Dimov, A [1 ]
Vassileva, N [1 ]
机构
[1] INST CHEM TECHNOL & BIOTECHNOL,RAZGRAD 7200,BULGARIA
关键词
membrane modification; immobilization; glucose oxidase; membrane potential; charge density;
D O I
10.1016/0376-7388(96)00043-9
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
The membrane potential and charge density of membranes based on acrylonitrile copolymer were studied before and after immobilization of glucose oxidase. Membranes surface modified with sodium hydroxide, hydroxylamine, hydrazine hydrate, dimethylaminoethyl methacrylate and 2-acrylamido-2-methylpropanesulfonic acid were used, as well as membranes prepared from acrylonitrile copolymer modified in bulk with sodium hydroxide and hydroxylamine. The membrane potential was found to be proportional to the amount of the ionogenic groups in the modified membranes. The effects of electrolyte type, concentration and pH of the NaCl solution on the membrane potential were studied. The charge densities of modified membranes before and after immobilization of glucose oxidase were calculated. A correlation between the amount of bound protein and membrane potential was found. The influence of substrate-glucose concentration on membrane potential of enzyme bound membranes was studied.
引用
收藏
页码:273 / 278
页数:6
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