JNK activation is not required for Fas-mediated apoptosis

被引：27

作者：

Low, W

Smith, A

Ashworth, A

Collins, M

机构：

[1] UCL, Dept Immunol, London W1P 6DB, England

[2] Inst Canc Res, Chester Beatty Labs, Sect Gene Funct & Regulat, London SW3 6JB, England

来源：

ONCOGENE | 1999年 / 18卷 / 25期

基金：

英国惠康基金;

关键词：

caspase; CD95; FADD; M3/6; IL-3;

D O I：

10.1038/sj.onc.1202702

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fas ligation in the presence of cycloheximide induced Jun N-terminal kinase 1 (JNK1) and JNK2 phosphorylation, caspase activation and cell death in the IL-3-dependent cell line BAF3. Fas-mediated apoptosis was prevented by expression of dominant negative FADD but not inhibited by IL-3. To investigate the role of JNK activation in this process, we examined cells overexpressing a JNK-specific phosphatase M3/6. M3/6 prevented Pas stimulation of JNK, but did not affect Pas-mediated caspase activation or cell death, demonstrating that JNK activation is not required for these processes.

引用

页码：3737 / 3741

页数：5

共 43 条

[1] Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death [J].