Mechanism and specificity of RNA cleavage by chemical ribonucleases.

被引：7

作者：

Beloglazova, N

Vlassov, A

Konevetc, D

Sil'nikov, V

Zenkova, M

Giege, R

Vlassov, V

机构：

[1] Russian Acad Sci, Inst Bioorgan Chem, Novosibirsk 630090, Russia

[2] CNRS, Inst Biol Mol & Cellulaire, Strasbourg, France

来源：

NUCLEOSIDES & NUCLEOTIDES | 1999年 / 18卷 / 6-7期

关键词：

D O I：

10.1080/07328319908044753

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cleaving of model RNA substrates by chemical ribonucleases constructed by conjugation of 1,4 diazabicyclo[2,2,2]octane with histamine and histidine was investigated. Similarly to RNase A, the chemical RNases produce fragments with 5' hydroxy-group and 3'-cyclophosphate. The cleavage occurs as the catalytic reaction: more than 150 phosphodiester bonds in RNA can be cleaved by one molecule of RNase mimic.

引用

页码：1463 / 1465

页数：3