Vaspin attenuates the apoptosis of human osteoblasts through ERK signaling pathway

被引：82

作者：

Zhu, Xiao ^{[1
,2
]}

Jiang, Yi ^{[3
]}

Shan, Peng-Fei ^{[4
]}

Shen, Jie ^{[2
]}

Liang, Qiu-Hua ^{[1
]}

Cui, Rong-Rong ^{[1
]}

Liu, Yuan ^{[1
]}

Liu, Guan-Ying ^{[1
]}

Wu, Shan-Shan ^{[1
]}

Lu, Qiong ^{[5
]}

Xie, Hui ^{[1
]}

Liu, You-Shuo ^{[6
]}

Yuan, Ling-Qing ^{[1
]}

Liao, Er-Yuan ^{[1
]}

机构：

[1] Cent S Univ, Xiang Ya Hosp 2, Inst Metab & Endocrinol, Changsha, Hunan, Peoples R China

[2] So Med Univ, Affiliated Hosp 3, Dept Metab & Endocrinol, Guangzhou 510630, Guangdong, Peoples R China

[3] Cent S Univ, Xiang Ya Hosp 2, Dept Pathol, Changsha, Hunan, Peoples R China

[4] Zhejiang Univ, Coll Med, Affiliated Hosp 2, Dept Endocrinol & Metab, Hangzhou 310003, Zhejiang, Peoples R China

[5] Cent S Univ, Xiang Ya Hosp 2, Inst Clin Pharm & Pharmacol, Changsha, Hunan, Peoples R China

[6] Cent S Univ, Xiang Ya Hosp 2, Dept Geriatr, Changsha, Hunan, Peoples R China

来源：

AMINO ACIDS | 2013年 / 44卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Vaspin; Osteoblast; Apoptosis; Extracellular signal-regulated kinase; GENE-EXPRESSION; BONE; LEPTIN; CELLS; INSULIN; PROLIFERATION; GHRELIN; APELIN; ADIPONECTIN; WOMEN;

D O I：

10.1007/s00726-012-1425-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

It has been hypothesized that adipocytokines originating from adipose tissue may have an important role in bone metabolism. Vaspin is a novel adipocytokine isolated from visceral white adipose tissue, which has been reported to have anti-apoptotic effects in vascular endothelial cells. However, to the best of our knowledge there is no information regarding the effects of vaspin on osteoblast apoptosis. This study therefore examined the possible effects of vaspin on apoptosis in human osteoblasts (hOBs). Our study established that vaspin inhibits hOBs apoptosis induced by serum deprivation, as determined by ELISA and TUNEL assays. Western blot analysis revealed that vaspin upregulates the expression of Bcl-2 and downregulates that of Bax in a dose-dependent manner. Vaspin stimulated the phosphorylation of ERK, and pretreatment of hOBs with the ERK inhibitor PD98059 blocked the vaspin-induced activation of ERK, however, vaspin did not stimulate the phosphorylation of p38, JNK or Akt. Vaspin protects hOBs from serum deprivation-induced apoptosis, which may be mediated by activating the MAPK/ERK signaling pathway.

引用

页码：961 / 968

页数：8

共 46 条

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