Early hematopoietic lineage restrictions directed by Ikaros

被引:234
作者
Yoshida, T
Ng, SYM
Zuniga-Pflucker, JC
Georgopoulos, K [1 ]
机构
[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Cutaneous Biol Res Ctr, Charlestown, MA 02129 USA
[2] Univ Toronto, Sunnybrook & Womens Coll Hlth Sci Ctr, Dept Immunol, Toronto, ON M4N 3M5, Canada
关键词
D O I
10.1038/ni1314
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Ikaros is expressed in early hematopoietic progenitors and is required for lymphoid differentiation. In the absence of Ikaros, there is a lack of markers defining fate restriction along lympho-myeloid pathways, but it is unclear whether formation of specific progenitors or expression of their markers is affected. Here we use a reporter based on Ikaros regulatory elements to separate early progenitors in wild-type and Ikaros-null mice. We found previously undetected Ikaros-null lympho-myeloid progenitors lacking the receptor tyrosine kinase Flt3 that were capable of myeloid but not lymphoid differentiation. In contrast, lack of Ikaros in the common myeloid progenitor resulted in increased formation of erythro-megakaryocytes at the expense of myeloid progenitors. Using this approach, we identify previously unknown pivotal functions for Ikaros in distinct fate 'decisions' in the early hematopoietic hierarchy.
引用
收藏
页码:382 / 391
页数:10
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