RAC protein directs the complete removal of the 3′ external transcribed spacer by the Pac1 nuclease

被引：14

作者：

Spasov, K ^{[1
]}

Perdomo, LI ^{[1
]}

Evakine, E ^{[1
]}

Nazar, RN ^{[1
]}

机构：

[1] Univ Guelph, Dept Mol Biol & Genet, Guelph, ON N1G 2W1, Canada

来源：

MOLECULAR CELL | 2002年 / 9卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1016/S1097-2765(02)00461-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Schizosaccharomyces pombe, interdependency in rRNA processing is mediated by a large protein complex (RAC) which contains independent binding sites for each of the transcribed spacers. The RAC complex exhibits no nuclease activity but dramatically alters the efficiency and specificity of the Pac1 nuclease, leading to the complete removal of the 3'ETS. Furthermore, the affinity of RAC protein for mutant 3'ETS correlates closely with in vivo effects on rRNA processing, and changes which disrupt RAC protein binding also inhibit Pac1 nuclease cleavage at the 3' end of the 25S rRNA sequence. The observations indicate that, in the presence of the RAC protein/3'ETS complex, cleavage by the RNase III-like homolog is not restricted to the known intermediate sites but also is directed at the 3' end of the 25S rRNA.

引用

页码：433 / 437

页数：5

共 26 条

[1] RNase III cleaves eukaryotic preribosomal RNA at a U3 snoRNP-dependent site [J].

AbouElela, S ;

Igel, H ;

Ares, M .

CELL, 1996, 85 (01) :115-124

[2]

Chaconas G, 1980, Methods Enzymol, V65, P75

[3]

COURT D, 1993, CONTROL MRNA STABILI, P70

[4] T7 EARLY RNAS AND ESCHERICHIA-COLI RIBOSOMAL-RNAS ARE CUT FROM LARGE PRECURSOR RNAS IN-VIVO BY RIBONUCLEASE-III [J].

DUNN, JJ ;

STUDIER, FW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (12) :3296-3300

[5] Interdependence in the processing of ribosomal RNAs in Schizosaccharomyces pombe [J].

Good, L ;

Intine, RVA ;

Nazar, RN .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 273 (04) :782-788

[6] LOCALIZATION OF AN ENDONUCLEASE SPECIFIC FOR DOUBLE-STRANDED-RNA WITHIN THE NUCLEOLUS AND ITS IMPLICATION IN PROCESSING RIBOSOMAL TRANSCRIPTS [J].

GRUMMT, I ;

HALL, SH ;

CROUCH, RJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1979, 94 (02) :437-443

[7] Structural features in the 3′ external transcribed spacer affecting intragenic processing of yeast rRNA [J].

Hitchen, J ;

Ivakine, E ;

Melekhovets, YF ;

Lalev, A ;

Nazar, RN .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 274 (04) :481-490

[8] Essential structural features in the Schizosaccharomyces pombe pre-rRNA 5′ external transcribed spacer [J].

Intine, RVA ;

Good, L ;

Nazar, RN .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 286 (03) :695-708

[9] Yeast Rnt1p is required for cleavage of the pre-ribosomal RNA in the 3′ ETS but not the 5′ ETS [J].

Kufel, J ;

Dichtl, B ;

Tollervey, D .

RNA, 1999, 5 (07) :909-917

[10] Structural equivalence in the transcribed spacers of pre-rRNA transcripts in Schizosaccharomyces pombe [J].

Lalev, AI ;

Nazar, RN .

NUCLEIC ACIDS RESEARCH, 1999, 27 (15) :3071-3078

← 1 2 3 →