The need for transparency and good practices in the qPCR literature

被引：217

作者：

Bustin, Stephen A. ^{[1
]}

Benes, Vladimir ^{[2
]}

Garson, Jeremy ^{[3
]}

Hellemans, Jan ^{[4
]}

Huggett, Jim ^{[5
]}

Kubista, Mikael ^{[6
]}

Mueller, Reinhold ^{[7
]}

Nolan, Tania ^{[8
]}

Pfaffl, Michael W. ^{[9
]}

Shipley, Gregory ^{[10
]}

Wittwer, Carl T. ^{[11
]}

Schjerling, Peter ^{[12
]}

Day, Philip J. ^{[13
,14
]}

Abreu, Monica ^{[15
]}

Aguado, Begona ^{[16
]}

Beaulieu, Jean-Francois ^{[17
]}

Beckers, Anneleen ^{[18
]}

Bogaert, Sara ^{[19
]}

Browne, John A. ^{[20
]}

Carrasco-Ramiro, Fernando ^{[16
]}

Ceelen, Liesbeth ^{[21
]}

Ciborowski, Kate ^{[22
]}

Cornillie, Pieter ^{[23
]}

Coulon, Stephanie ^{[24
]}

Cuypers, Ann ^{[25
]}

De Brouwer, Sara ^{[18
]}

De Ceuninck, Leentje ^{[26
]}

De Craene, Jurgen ^{[23
]}

De Naeyer, Helene ^{[27
]}

De Spiegelaere, Ward ^{[28
]}

Deckers, Kato ^{[29
]}

Dheedene, Annelies ^{[18
]}

Durinck, Kaat ^{[18
]}

Ferreira-Teixeira, Margarida ^{[15
]}

Fieuw, Annelies ^{[18
]}

Gallup, Jack M. ^{[30
]}

Gonzalo-Flores, Sandra ^{[16
]}

Goossens, Karen ^{[31
]}

Heindryckx, Femke ^{[32
]}

Herring, Elizabeth ^{[17
]}

Hoenicka, Hans ^{[33
]}

Icardi, Laura ^{[26
]}

Jaggi, Rolf ^{[34
]}

Javad, Farzad ^{[13
,14
]}

Karampelias, Michael ^{[35
]}

Kibenge, Frederick ^{[36
]}

Kibenge, Molly ^{[36
]}

Kumps, Candy ^{[18
]}

Lambertz, Irina ^{[18
]}

Lammens, Tim ^{[37
]}

机构：

[1] Anglia Ruskin Univ, Postgrad Med Inst, Chelmsford, Essex, England

[2] EMBL, Sci Core Facil, Heidelberg, Germany

[3] UCL, Div Infect & Immun, London, England

[4] Biogazelle NV, Zwijnaarde, Belgium

[5] Mol & Cell Biol LGC, Teddington, Middx, England

[6] TATAA Bioctr, Gothenburg, Sweden

[7] RM Consulting, San Diego, CA USA

[8] Sigma, Tech Support, Haverhill, MA USA

[9] Tech Univ Munich, Physiol Unit, Freising Weihenstephan, Germany

[10] Shipley Consulting, Austin, TX USA

[11] Univ Utah, Dept Pathol, Salt Lake City, UT USA

[12] Bispebjerg Hosp, Inst Sports Med, DK-2400 Copenhagen, Denmark

[13] Univ Manchester, Fac Med, Manchester, Lancs, England

[14] Univ Manchester, Manchester Inst Biotechnol, Manchester, Lancs, England

[15] Univ Coimbra, Fac Med, Coimbra, Portugal

[16] UAM, CSIC, Ctr Biol Mol Severo Ochoa, Madrid, Spain

[17] Univ Sherbrooke, Fac Med & Hlth Sci, Sherbrooke, PQ J1K 2R1, Canada

[18] Univ Ghent, Ctr Med Genet, B-9000 Ghent, Belgium

[19] Univ Ghent, Dept Gastroenterol, B-9000 Ghent, Belgium

[20] Univ Coll Dublin, Sch Agr & Food Sci, Dublin 2, Ireland

[21] Pathlicon NV, Evergem, Belgium

[22] Univ Bristol, Sch Biol Sci, Bristol, Avon, England

[23] Univ Ghent, Dept Morphol, Merelbeke, Belgium

[24] Univ Ghent, Dept Gastroenterol & Hepatol, B-9000 Ghent, Belgium

[25] Hasselt Univ, Ctr Environm Sci, Diepenbeek, Belgium

[26] Univ Ghent VIB, Dept Med Prot Res, B-9052 Ghent, Belgium

[27] Univ Ghent, Dept Movement & Sports Sci, B-9000 Ghent, Belgium

[28] Univ Ghent, Dept Internal Med, B-9000 Ghent, Belgium

[29] Univ Louvain, Ctr Mol & Vasc Biol, Louvain, Belgium

[30] Iowa State Univ, Dept Vet Pathol, Ames, IA USA

[31] Univ Ghent, Dept Nutr Genet & Ethol, Merelbeke, Belgium

[32] Uppsala Univ, Dept Med Biochem & Microbiol, Uppsala, Sweden

[33] Thunen Inst Forest Genet, Grosshansdorf, Germany

[34] Univ Bern, Dept Clin Res, Bern, Switzerland

[35] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[36] Univ Prince Edward Isl, Atlantic Vet Coll, Charlottetown, PE C1A 4P3, Canada

[37] Ghent Univ Hosp, Dept Pediat Hematol Oncol & Stem Cell Transplanta, Ghent, Belgium

[38] Ghent Univ Hosp, Dept Gen Internal Med & Infect Dis, Ghent, Belgium

[39] IRTA, San Carlos de la Rapita, Spain

[40] Inst Trop Med, Dept Biomed Sci, B-2000 Antwerp, Belgium

[41] Univ Ghent, Dept Reprod Obstet & Herd Hlth Management, B-9000 Ghent, Belgium

[42] Natl Inst Nutr & Seafood Res, Bergen, Norway

[43] Ghent Univ Hosp, Dept Otorhinolaryngol, Ghent, Belgium

[44] Charite, Julius Wolff Inst, D-13353 Berlin, Germany

[45] Tech Univ Denmark, Natl Vet Inst, Frederiksberg, Denmark

[46] Med Univ Vienna, Dept Orthopaed, Vienna, Austria

[47] Univ Ghent, Dept Dermatol, B-9000 Ghent, Belgium

[48] Sigma Aldrich Sweden AB, Stockholm, Sweden

[49] Sanford Burnham Med Res Inst, Muscle Dev & Regenerat Program, La Jolla, CA USA

[50] Ctr Integrat Biol CIBIO, Lab Transcript Networks, Trento, Italy

来源：

NATURE METHODS | 2013年 / 10卷 / 11期

关键词：

POLYMERASE CHAIN-REACTION; REAL-TIME; QUANTITATIVE PCR; RNA; QUANTIFICATION; NORMALIZATION; PUBLICATION; EXPRESSION;

D O I：

10.1038/nmeth.2697

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

引用

页码：1063 / 1067

页数：5

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