AtE2F-a and AtDP-a, members of the E2F family of transcription factors, induce Arabidopsis leaf cells to re-enter S phase

被引:50
作者
Rossignol, P
Stevens, R
Perennes, C
Jasinski, S
Cella, R
Tremousaygue, D
Bergounioux, C [1 ]
机构
[1] Univ Paris 11, CNRS, UMR 8618, Inst BIotechnol Plantes, F-91405 Orsay, France
[2] Univ Pavia, Dept Genet & Microbiol, I-27100 Pavia, Italy
[3] INRA, CNRS, Lab Biol Mol Relat Plantes Microorgismes, F-31326 Castanet Tolosan, France
关键词
E2F transcription factors; Arabidopsis; S phase induction; cell cycle; transient expression;
D O I
10.1007/s00438-001-0624-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In eukaryotes, transcription factors of the E2F family, in addition to having a role in cell proliferation, participate in regulating apoptosis, differentiation and development. In Arabidopsis thaliana, eight gene sequences have been identified as encoding E2F or DP homologues. DP proteins form heterodimers with E2Fs. The aim of this work was to characterize the functions of three of these factors: AtE2F-a, AtE2F-b and AtDP-a. Here we report that AtE2F-a and AtE2F-b transactivate a reporter gene via an E2F consensus cis-acting element in Arabidopsis protoplasts. AtE2F-a is a more potent activator than AtE2F-b. Furthermore, co-expression of the E2F partner AtDP-a, or the DNA binding protein AtPuralpha, modulates the activation of AtE2F-alpha. Taken together, these results suggest that AtE2F-a, AtE2F-b and AtDP-a share features characteristic of members of the E2F family of transcription factors. Moreover, overexpression of AtE2F-alpha and AtDP-a can induce differentiated, non-dividing, leaf cells to re-enter S-phase. We conclude that the transcription factor AtE2F-a plays an important role in progression into S phase, which probably correlates with its capacity to stimulate transcription.
引用
收藏
页码:995 / 1003
页数:9
相关论文
共 34 条
[1]   DcE2F, a functional plant E2F-like transcriptional activator from Daucus carota [J].
Albani, D ;
Mariconti, L ;
Ricagno, S ;
Pitto, L ;
Moroni, C ;
Helin, K ;
Cella, R .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (25) :19258-19267
[2]  
AXELOS M, 1992, PLANT PHYSIOL BIOCH, V30, P123
[3]  
BERGOUNIOUX C, 1992, PHYSIOL PLANTARUM, V85, P374, DOI 10.1111/j.1399-3054.1992.tb04750.x
[4]   Cell cycle regulation of the tobacco ribonucleotide reductase small subunit gene is mediated by E2F-like elements [J].
Chabouté, ME ;
Clément, B ;
Sekine, M ;
Philipps, G ;
Chaubet-Gigot, N .
PLANT CELL, 2000, 12 (10) :1987-1999
[5]   GENOMIC SEQUENCING [J].
CHURCH, GM ;
GILBERT, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995
[6]   Association of Purα and E2F-1 suppresses transcriptional activity of E2F-1 [J].
Darbinian, N ;
Gallia, GL ;
Kundu, M ;
Shcherbik, N ;
Tretiakova, A ;
Giordano, A ;
Khalili, K .
ONCOGENE, 1999, 18 (46) :6398-6402
[7]   Integration of a growth-suppressing BTB/POZ domain protein with the DP component of the E2F transcription factor [J].
de la Luna, S ;
Allen, KE ;
Mason, SL ;
La Thangue, NB .
EMBO JOURNAL, 1999, 18 (01) :212-228
[8]   Cell cycling and cell enlargement in developing leaves of Arabidopsis [J].
Donnelly, PM ;
Bonetta, D ;
Tsukaya, H ;
Dengler, RE ;
Dengler, NG .
DEVELOPMENTAL BIOLOGY, 1999, 215 (02) :407-419
[9]   Proliferating cell nuclear antigen transcription is repressed through an E2F consensus element and activated by geminivirus infection in mature leaves [J].
Egelkrout, EM ;
Robertson, D ;
Hanley-Bowdoin, L .
PLANT CELL, 2001, 13 (06) :1437-1452
[10]   OLOMOUCINE, AN INHIBITOR OF THE CDC2/CDK2 KINASES ACTIVITY, BLOCKS PLANT-CELLS AT THE G1 TO S AND G2 TO M CELL-CYCLE TRANSITIONS [J].
GLAB, N ;
LABIDI, B ;
QIN, LX ;
TREHIN, C ;
BERGOUNIOUX, C ;
MEIJER, L .
FEBS LETTERS, 1994, 353 (02) :207-211