Crystallization of recombinant Crithidia fasciculata tryparedoxin

被引：6

作者：

Alphey, MS ^{[1
]}

Tetaud, E ^{[1
]}

Gourley, DG ^{[1
]}

Fairlamb, AH ^{[1
]}

Hunter, WN ^{[1
]}

机构：

[1] Univ Dundee, Dept Biochem, Dundee DD1 5EH, Scotland

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1999年 / 126卷 / 01期

基金：

英国惠康基金;

关键词：

Crithidia fasciculata; thioredoxin; tryparedoxin; crystallization; X-ray diffraction;

D O I：

10.1006/jsbi.1999.4091

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant tryparedoxin, a thioredoxin homologue from Crithidia fasciculata, has been purified from an Escherichia coli expression system and used in crystallization trials. Orthorhombic needles in space group P2(1)2(1)2(1) with unit cell dimensions of a = 38.63, b = 51.47, and c = 73.41 Angstrom, have been obtained. The crystals present a monomer of approximate molecular mass 16 kDa in the asymmetric unit and diffract to 1.8-Angstrom resolution using synchrotron radiation. Structure determination will be carried out to further the understanding of the; role tryparedoxin plays in regulating oxidative stress in parasitic trypanosomatids. (C) 1999 Academic Press.

引用

页码：76 / 79

页数：4

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[3]

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[4]

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