Profiling lipid changes in plant response to low temperatures

被引：47

作者：

Wang, XM ^{[1
]}

Li, WQ

Li, MY

Welti, R

机构：

[1] Univ Missouri, Dept Biol, St Louis, MO 63121 USA

[2] Donald Danforth Plant Sci Ctr, St Louis, MO USA

[3] Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China

[4] Kansas State Univ, Dept Biochem, Manhattan, KS USA

[5] Kansas State Univ, Div Biol, Manhattan, KS USA

来源：

PHYSIOLOGIA PLANTARUM | 2006年 / 126卷 / 01期

关键词：

D O I：

10.1111/j.1399-3054.2006.00622.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Changes in membrane lipid composition play multiple roles in plant adaptation and survival in the face of chilling and freezing damage. An electrospray ionization tandem mass spectrometry (ESI-MS/MS)-based approach has been used to quantitatively profile membrane lipid molecular species in plant response to low temperatures. This method involves the direct infusion of unfractionated lipid extracts into a mass spectrometer in the precursor and neutral loss scanning modes to identify and quantify lipid species. The profiling analysis reveals significant and distinct lipid changes during cold acclimation and freezing. Comparative profiling of wildtype and mutants provides information about the metabolic and cellular functions of specific phospholipase D genes and enzymes.

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收藏

页码：90 / 96

页数：7

相关论文

共 20 条

[1]

Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry [J].

Brugger, B ;

Erben, G ;

Sandhoff, R ;

Wieland, FT ;

Lehmann, WD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2339-2344

[2]

A 90-kD phospholipase D from tobacco binds to microtubules and the plasma membrane [J].

Gardiner, JC ;

Harper, JDI ;

Weerakoon, ND ;

Collings, DA ;

Ritchie, S ;

Gilroy, S ;

Cyr, RJ ;

Marc, J .

PLANT CELL, 2001, 13 (09) :2143-2158

[3]

Shotgun lipidomics: Electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples [J].

Han, XL ;

Gross, RW .

MASS SPECTROMETRY REVIEWS, 2005, 24 (03) :367-412

[4]

Acclimative response to temperature stress in higher plants: Approaches of gene engineering for temperature tolerance [J].

Iba, K .

ANNUAL REVIEW OF PLANT BIOLOGY, 2002, 53 :225-245

[5]

Involvement of a novel Arabidopsis phospholipase D, AtPLDδ, in dehydration-inducible accumulation of phosphatidic acid in stress signalling [J].

Katagiri, T ;

Takahashi, S ;

Shinozaki, K .

PLANT JOURNAL, 2001, 26 (06) :595-605

[6]

The plasma membrane-bound phospholipase Dδ enhances freezing tolerance in Arabidopsis thaliana [J].

Li, WQ ;

Li, MY ;

Zhang, WH ;

Welti, R ;

Wang, XM .

NATURE BIOTECHNOLOGY, 2004, 22 (04) :427-433

[7]

Murphy M F, 1993, Arch Psychiatr Nurs, V7, P226, DOI 10.1016/0883-9417(93)90031-Q

[8]

Substrate selectivities and lipid modulation of plant phospholipase Dα, -β, and -γ [J].

Pappan, K ;

Austin-Brown, S ;

Chapman, KD ;

Wang, XM .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1998, 353 (01) :131-140

[9]

Kinetic analysis of Arabidopsis phospholipase Dδ -: Substrate preference and mechanism of activation by Ca2+ and phosphatidylinositol 4,5-bisphosphate [J].

Qin, CB ;

Wang, CX ;

Wang, XM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (51) :49685-49690

[10]

Activation of phospholipases C and D is an early response to a cold exposure in Arabidopsis suspension cells [J].

Ruelland, E ;

Cantrel, C ;

Gawer, M ;

Kader, JC ;

Zachowski, A .

PLANT PHYSIOLOGY, 2002, 130 (02) :999-1007