miR-24-3p promotes cell migration and proliferation in lung cancer by targeting SOX7

被引：94

作者：

Yan, Liang ^{[1
,2
]}

Ma, Jinzhu ^{[2
]}

Zhu, Yiping ^{[3
]}

Zan, Jiawei ^{[1
]}

Wang, Zhen ^{[3
]}

Ling, Liefeng ^{[2
]}

Li, Qiang ^{[2
]}

Lv, Jun ^{[2
]}

Qi, Shimei ^{[2
]}

Cao, Yingya ^{[3
]}

Liu, Ying ^{[2
]}

Cao, Long ^{[2
]}

Zhang, Yao ^{[2
]}

Qi, Zhilin ^{[2
]}

Nie, Liuwang ^{[1
]}

机构：

[1] Anhui Normal Univ, Sch Life Sci, Prov Key Lab Conservat & Exploitat Res Biol Resou, Wuhu, Anhui, Peoples R China

[2] Wannan Med Coll, Prov Key Lab Biol Macromol Res, Wuhu, Anhui, Peoples R China

[3] Wannan Med Coll, Affiliated Hosp 1, Wuhu, Anhui, Peoples R China

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2018年 / 119卷 / 05期

基金：

中国国家自然科学基金;

关键词：

lung cancer; miR-24-3p; SOX7; TUMOR-SUPPRESSOR; INVASION; METASTASIS; CARCINOMA; APOPTOSIS; PATHWAY; GROWTH;

D O I：

10.1002/jcb.26553

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Lung cancer (LC) is one of the leading causes of cancer-related death in the world. miR-24-3p plays critical roles in many cancer types, including LC. In this study, we first investigated whether miR-24-3p promoted LC cell migration and proliferation in vitro. We used three bioinformatics algorithms to predict the miR-24-3p target gene to study the molecular mechanism by which miR-24-3p contributes to LC progression. Then, we used the luciferase reporter assay to identify whether SOX7 was a direct target of miR-24-3p. Moreover, Western blotting and a quantitative real time-polymerase chain reaction analysis showed that miR-24-3p downregulated SOX7 protein expression by a post-transcriptional mechanism. Finally, we determined that SOX7 had opposing effects to those of miR-24-3p on LC cell proliferation and migration, suggesting that miR-24-3p promotes cell proliferation and migration by directly targeting SOX7. Furthermore, miR-24-3p accelerated tumor growth in xenograft mice by targeting SOX7. These results provide the first clue that miR-24-3p could play a role as an oncomiR in LC by regulating SOX7.

引用

页码：3989 / 3998

页数：10

共 29 条

[1]

Novel miRNA-mRNA interactions conserved in essential cancer pathways [J].