A novel superfamily of transporters for allantoin and other oxo derivatives of nitrogen heterocyclic compounds in Arabidopsis

被引:85
作者
Desimone, M
Catoni, E
Ludewig, U
Hilpert, M
Schneider, A
Kunze, R
Tegeder, M
Frommer, WB
Schumacher, K
机构
[1] Zentrum Mol Biol Pflazen, D-72076 Tubingen, Germany
[2] Univ Cologne, Bot Inst 2, D-50931 Cologne, Germany
[3] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA
关键词
D O I
10.1105/tpc.010458
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A wide spectrum of soil heterocyclic nitrogen compounds are potential nutrients for plants. Here, it is shown that Arabidopsis plants are able to use allantoin as sole nitrogen source. By functional complementation of a yeast mutant defective in allantoin uptake, an Arabidopsis transporter, AtUPS1 (Arabidopsis thaliana ureide permease 1), was identified. AtUPS1 belongs to a novel superfamily of plant membrane proteins with five open reading frames in Arabidopsis (identity, 64 to 82%). UPS proteins have 10 putative transmembrane domains with a large cytosolic central domain containing a "Walker A" motif. Transport of C-14-labeled allantoin by AtUPS1 in yeast exhibited saturation kinetics (K-m similar to 52 muM), was dependent on Glc and a proton gradient, and was stimulated by acidic pH. AtUPS1 transports uric acid and xanthine, besides allantoin, but not adenine. Protons are cosubstrates in allantoin transport by AtUPS1, as demonstrated by expression in Xenopus laevis oocytes. In plants, AtUPS1 gene expression was dependent on the nitrogen source. Therefore, AtUPS1 presumably is involved in the uptake of allantoin and other purine degradation products when primary sources are limiting.
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页码:847 / 856
页数:10
相关论文
共 26 条
[1]  
Altschul SF, 1996, METHOD ENZYMOL, V266, P460
[2]   Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues [J].
An, YQ ;
McDowell, JM ;
Huang, SR ;
McKinney, EC ;
Chambliss, S ;
Meagher, RB .
PLANT JOURNAL, 1996, 10 (01) :107-121
[3]   Functional characterization of a maize purine transporter by expression in Aspergillus nidulans [J].
Argyrou, E ;
Sophianopoulou, V ;
Schultes, N ;
Diallinas, G .
PLANT CELL, 2001, 13 (04) :953-964
[4]  
BERGMEYER HU, 1970, METHODEN ENZYMATISCH, V1, P478
[5]   REGULATION OF ALLANTOATE TRANSPORT IN WILD-TYPE AND MUTANT STRAINS OF SACCHAROMYCES-CEREVISIAE [J].
CHISHOLM, VT ;
LEA, HZ ;
RAI, R ;
COOPER, TG .
JOURNAL OF BACTERIOLOGY, 1987, 169 (04) :1684-1690
[6]   WHAT IS THE FUNCTION OF NITROGEN CATABOLITE REPRESSION IN SACCHAROMYCES-CEREVISIAE [J].
COOPER, TG ;
SUMRADA, RA .
JOURNAL OF BACTERIOLOGY, 1983, 155 (02) :623-627
[7]   PURINES AND PYRIMIDINES IN SOILS AND HUMIC SUBSTANCES [J].
CORTEZ, J ;
SCHNITZER, M .
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL, 1979, 43 (05) :958-961
[8]  
de Koning H, 2000, MOL MEMBR BIOL, V17, P75
[9]   A new family of high-affinity transporters for adenine, cytosine, and purine derivatives in arabidopsis [J].
Gillissen, B ;
Bürkle, L ;
André, B ;
Kühn, C ;
Rentsch, D ;
Brandl, B ;
Frommer, WB .
PLANT CELL, 2000, 12 (02) :291-300
[10]   UTILIZATION OF EXOGENOUS PYRIMIDINES AND RECYCYCLING OF URIDINE-5'-PHOSPHATE DERIVATIVES IN SACCHAROMYCES-CEREVISIAE, AS STUDIED BY MEANS OF MUTANTS AFFECTED IN PYRIMIDINE UPTAKE AND METABOLISM [J].
GRENSON, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 11 (02) :249-&