Hyaluronic acid-human blood hydrogels for stem cell transplantation

被引：55

作者：

Chang, Connie Y. ^{[2
]}

Chan, Angel T. ^{[1
]}

Armstrong, Patrick A. ^{[2
]}

Luo, Hong-Chang ^{[1
]}

Higuchi, Takahiro ^{[3
]}

Strehin, Iossif A. ^{[2
]}

Vakrou, Styliani ^{[1
]}

Lin, Xiaoping ^{[1
]}

Brown, Sophia N. ^{[1
]}

O'Rourke, Brian ^{[1
]}

Abraham, Theodore P. ^{[1
]}

Wahl, Richard L. ^{[3
]}

Steenbergen, Charles J. ^{[4
]}

Elisseeff, Jennifer H. ^{[2
]}

Abraham, M. Roselle ^{[1
]}

机构：

[1] Johns Hopkins Sch Med, Div Cardiovasc Med, Baltimore, MD 21205 USA

[2] Johns Hopkins Sch Med, Dept Biomed Engn, Baltimore, MD 21205 USA

[3] Johns Hopkins Sch Med, Dept Radiol, Baltimore, MD 21205 USA

[4] Johns Hopkins Sch Med, Dept Pathol, Baltimore, MD 21205 USA

来源：

BIOMATERIALS | 2012年 / 33卷 / 32期

关键词：

Bioadhesive and biodegradable hydrogel; Autologous blood hydrogel; Modified hyaluronic acid; Cardiac stem cell transplantation; Molecular imaging; Echocardiography; HEART; ANGIOGENESIS; IMPLANTATION; MYOCARDIUM; SCAFFOLD;

D O I：

10.1016/j.biomaterials.2012.07.058

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Tissue engineering-based approaches have the potential to improve stem cell engraftment by increasing cell delivery to the myocardium. Our objective was to develop and characterize a naturally-derived, autologous, biodegradable hydrogel in order to improve acute stem cell retention in the myocardium. HA-blood hydrogels (HA-BL) were synthesized by mixing in a 1:1(v/v) ratio, lysed whole blood and hyaluronic acid (HA), whose carboxyl groups were functionalized with N-hydroxysuccinimide (NHS) to yield HA succinimidyl succinate (HA NHS). We performed physical characterization and measured survival/proliferation of cardiosphere-derived cells (CDCs) encapsulated in the hydrogels. Hydrogels were injected intra-myocardially or applied epicardially in rats. NHS-activated carboxyl groups in HA react with primary amines present in blood and myocardium to form amide bonds, resulting in a 3D hydrogel bound to tissue. HA-blood hydrogels had a gelation time of 58 +/- 12s, swelling ratio of 10 +/- 0.5, compressive and elastic modulus of 14 +/- 3 and 1.75 +/- 0.6 kPa respectively. These hydrogels were not degraded at 4wks by hydrolysis alone. CDC encapsulation promoted their survival and proliferation. Intra-myocardial injection of CDCs encapsulated in these hydrogels greatly increased acute myocardial retention (p = 0.001). Epicardial application of HA-blood hydrogels improved left ventricular ejection fraction following myocardial infarction (p = 0.01). HA-blood hydrogels are highly adhesive, biodegradable, promote CDC survival and increase cardiac function following epicardial application after myocardial infarction. Published by Elsevier Ltd.

引用

页码：8026 / 8033

页数：8

共 39 条

[1]

Antiarrhythmic engineering of skeletal myoblasts for cardiac transplantation [J].

Abraham, MR ;

Henrikson, CA ;

Tung, L ;

Chang, MG ;

Aon, M ;

Xue, T ;

Li, RA ;

O'Rourke, B ;

Marbán, E .

CIRCULATION RESEARCH, 2005, 97 (02) :159-167

[2]

PicoGreen quantitation of DNA: Effective evaluation of samples pre- or post-PCR [J].

Ahn, SJ ;

Costa, J ;

Emanuel, JR .

NUCLEIC ACIDS RESEARCH, 1996, 24 (13) :2623-2625

[3]

Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures [J].

Almany, L ;

Seliktar, D .

BIOMATERIALS, 2005, 26 (15) :2467-2477

[4]

Cardiac Resynchronization by Cardiosphere-Derived Stem Cell Transplantation in an Experimental Model of Myocardial Infarction [J].

Bonios, Michael ;

Chang, Connie Y. ;

Pinheiro, Aurelio ;

Dimaano, Veronica Lea ;

Higuchi, Takahiro ;

Melexopoulou, Christina ;

Bengel, Frank ;

Terrovitis, John ;

Abraham, Theodore P. ;

Abraham, M. Roselle .

JOURNAL OF THE AMERICAN SOCIETY OF ECHOCARDIOGRAPHY, 2011, 24 (07) :808-814

[5]

Myocardial substrate and route of administration determine acute cardiac retention and lung bio-distribution of cardiosphere-derived cells [J].

Bonios, Michael ;

Terrovitis, John ;

Chang, Connie Y. ;

Engles, James M. ;

Higuchi, Takahiro ;

Lautamaeki, Riikka ;

Yu, Jianhua ;

Fox, James ;

Pomper, Martin ;

Wahl, Richard L. ;

Tsui, Benjamin M. ;

O'Rourke, Brian ;

Bengel, Frank M. ;

Marban, Eduardo ;

Abraham, M. Roselle .

JOURNAL OF NUCLEAR CARDIOLOGY, 2011, 18 (03) :443-450

[6]

Hyaluronic Acid Hydrogels for Biomedical Applications [J].

Burdick, Jason A. ;

Prestwich, Glenn D. .

ADVANCED MATERIALS, 2011, 23 (12) :H41-H56

[7]

Busher J.T., 1990, Clinical Methods: The History, Physical, and Laboratory Examinations, V3rd

[8]

Cecil R.L., 2000, CECIL TXB MED

[9]

Spiral Waves and Reentry Dynamics in an In Vitro Model of the Healed Infarct Border Zone [J].

Chang, Marvin G. ;

Zhang, Yibing ;

Chang, Connie Y. ;

Xu, Linmiao ;

Emokpae, Roland ;

Tung, Leslie ;

Marban, Eduardo ;

Abraham, M. Roselle .

CIRCULATION RESEARCH, 2009, 105 (11) :1062-U56

[10]

Relative Roles of Direct Regeneration Versus Paracrine Effects of Human Cardiosphere-Derived Cells Transplanted Into Infarcted Mice [J].

Chimenti, Isotta ;

Smith, Rachel Ruckdeschel ;

Li, Tao-Sheng ;

Gerstenblith, Gary ;

Messina, Elisa ;

Giacomello, Alessandro ;

Marban, Eduardo .

CIRCULATION RESEARCH, 2010, 106 (05) :971-U304

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