Mechanical properties of bacterial cellulose and interactions with smooth muscle cells

被引:438
作者
Bäckdahl, H
Helenius, G
Bodin, A
Nannmark, U
Johansson, BR
Risberg, B
Gatenholm, P [1 ]
机构
[1] Chalmers Univ Technol, Dept Biol & Chem Engn, SE-41296 Gothenburg, Sweden
[2] Sahlgrens Univ Hosp, Vasc Engn Ctr, Dept Surg, SE-41345 Gothenburg, Sweden
[3] Univ Gothenburg, Sahlgrens Univ Hosp, Electron Microscopy Unit, Dept Anat & Cell Biol, SE-40530 Gothenburg, Sweden
关键词
bacterial cellulose; scaffold; smooth muscle cell; tissue engineering blood vessels;
D O I
10.1016/j.biomaterials.2005.10.026
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Tissue engineered blood vessels (TEBV) represent an attractive approach for overcoming reconstructive problems associated with vascular diseases by providing small calibre vascular grafts. The aim of this study has been to evaluate a novel biomaterial, bacterial cellulose (BC), as a potential scaffold for TEBV. The morphology of the BC pellicle grown in static culture was investigated with SEM. Mechanical properties of BC were measured in Krebs solution and compared with the properties of porcine carotid arteries and ePTFE grafts. Attachment, proliferation and ingrowth of human smooth muscle cells (SMC) on the BC were analysed in vitro. The BC pellicle had an asymmetric structure composed of a fine network of nanofibrils similar to a collagen network. The shape of the stress-strain response of BC is reminiscent of the stress-strain response of the carotid artery, most probably due to the similarity in architecture of the nanofibrill networks. SMC adhered to and proliferated on the BC pellicle, an ingrowth of up to 40 mu m was seen after 2 weeks of culture. BC exhibit attractive properties for use in future TEBV. (C) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2141 / 2149
页数:9
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