The GTPase elongation factor ( EF)-G is responsible for promoting the translocation of the messenger RNA-transfer RNA complex on the ribosome, thus opening up the A site for the next aminoacyl-tRNA. Chemical modification and cryo-EM studies have indicated that tRNAs can bind the ribosome in an alternative 'hybrid' state after peptidyl transfer and before translocation, though the relevance of this state during translation elongation has been a subject of debate. Here, using pre-steady-state kinetic approaches and mutant analysis, we show that translocation by EF-G is most efficient when tRNAs are bound in a hybrid state, supporting the argument that this state is an authentic intermediate during translation.
机构:
Univ Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USA
Fredrick, K
;
Noller, HF
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Univ Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USA
机构:
Univ Calif Santa Cruz, Ctr Biol Mol, RNA Sinsheimer Labs, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Ctr Biol Mol, RNA Sinsheimer Labs, Santa Cruz, CA 95064 USA
Fredrick, K
;
Noller, HF
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Santa Cruz, Ctr Biol Mol, RNA Sinsheimer Labs, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Ctr Biol Mol, RNA Sinsheimer Labs, Santa Cruz, CA 95064 USA
机构:
Univ Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USA
Fredrick, K
;
Noller, HF
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USA
机构:
Univ Calif Santa Cruz, Ctr Biol Mol, RNA Sinsheimer Labs, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Ctr Biol Mol, RNA Sinsheimer Labs, Santa Cruz, CA 95064 USA
Fredrick, K
;
Noller, HF
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Santa Cruz, Ctr Biol Mol, RNA Sinsheimer Labs, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Ctr Biol Mol, RNA Sinsheimer Labs, Santa Cruz, CA 95064 USA