Studies on the role of the metK gene product of Escherichia coli K-12

被引:52
作者
Wei, YH [1 ]
Newman, EB [1 ]
机构
[1] Concordia Univ, Dept Biol, Montreal, PQ H3G 1M8, Canada
关键词
D O I
10.1046/j.1365-2958.2002.02856.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We show here that the metK gene is essential to the growth of Escherichia coli K-12 and can be deleted only in the presence of a rescue plasmid carrying a functional metK gene. When metK expression was limited, genomic DNA methylation decreased and cell division was hampered. Through primer extension, the transcription start site of metK was located at 140 bp upstream of the translation start site. The frequently used metK,,4 mutant has been shown to carry an A(r)G transition in the -10 region of the metK promoter. This accounts for its low level of S-adenosylmethionine (SAM) synthetase and SAM deficiency.
引用
收藏
页码:1651 / 1656
页数:6
相关论文
共 24 条
[1]  
[Anonymous], 1996, E COLI SALMONELLA CE
[2]   Functional characterization of a novel serotonin receptor (5-HTap2) expressed in the CNS of Aplysia californica [J].
Barbas, D ;
Zappulla, JP ;
Angers, S ;
Bouvier, M ;
Castellucci, VF ;
DesGroseillers, L .
JOURNAL OF NEUROCHEMISTRY, 2002, 80 (02) :335-345
[3]   mraW, an essential gene at the dcw cluster of Escherichia coli codes for a cytoplasmic protein with methyltransferase activity [J].
Carrión, M ;
Gómez, MJ ;
Merchante-Schubert, R ;
Dongarrá, S ;
Ayala, JA .
BIOCHIMIE, 1999, 81 (8-9) :879-888
[4]   Comparison of the sensitivities of two Escherichia coli genes to in vivo variation of lrp concentration [J].
Chen, CF ;
Newman, EB .
JOURNAL OF BACTERIOLOGY, 1998, 180 (03) :655-659
[5]  
Cheng X.D., 1999, S-Adenosylmethionine-Dependent Methyltransferases: Structures and Functions
[6]  
Greene RC., 1996, Escherichia coli and Salmonella, P542
[7]   TIGHT REGULATION, MODULATION, AND HIGH-LEVEL EXPRESSION BY VECTORS CONTAINING THE ARABINOSE P-BAD PROMOTER [J].
GUZMAN, LM ;
BELIN, D ;
CARSON, MJ ;
BECKWITH, J .
JOURNAL OF BACTERIOLOGY, 1995, 177 (14) :4121-4130
[8]   REGULATION OF S-ADENOSYLMETHIONINE SYNTHETASE IN ESCHERICHIA-COLI [J].
HOLLOWAY, CT ;
GREENE, RC ;
SU, CH .
JOURNAL OF BACTERIOLOGY, 1970, 104 (02) :734-&
[9]   EXPRESSION OF THE CLONED COLIPHAGE T3 S-ADENOSYLMETHIONINE HYDROLASE GENE INHIBITS DNA METHYLATION AND POLYAMINE BIOSYNTHESIS IN ESCHERICHIA-COLI [J].
HUGHES, JA ;
BROWN, LR ;
FERRO, AJ .
JOURNAL OF BACTERIOLOGY, 1987, 169 (08) :3625-3632
[10]  
LIN RT, 1990, J BACTERIOL, V172, P4529