DETERMINATION OF ZINC USING CARBONIC ANHYDRASE-BASED FLUORESCENCE BIOSENSORS

被引：27

作者：

Bozym, Rebecca ^{[1
,2
]}

Hurst, Tamiika K. ^{[3
]}

Westerberg, Nissa ^{[1
,4
]}

Stoddard, Andrea ^{[3
]}

Fierke, Carol A. ^{[3
]}

Frederickson, Christopher J. ^{[5
]}

Thompson, Richard B. ^{[1
]}

机构：

[1] Univ Maryland, Sch Med, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA

[2] Cellumen Inc, Pittsburgh, PA USA

[3] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

[4] US Patent & Trademark Off, Alexandria, VA USA

[5] NeuroBioTex Inc, Galveston, TX USA

来源：

FLUORESCENCE SPECTROSCOPY | 2008年 / 450卷

关键词：

D O I：

10.1016/S0076-6879(08)03414-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This chapter summarizes the use of carbonic anhydrase (CA)-based fluorescent indicators to determine free zinc in solution, in cells, and in subcellular organelles. Expression (both in situ and in vitro) and preparation of CA-based indicators are described, together with techniques of their use, and procedures to minimize contamination. Recipes for zinc buffers are supplied.

引用

页码：287 / 309

页数：23

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