GmTMT2a from soybean elevates the α-tocopherol content in corn and Arabidopsis

被引：32

作者：

Zhang, Lan ^{[1
]}

Luo, Yanzhong ^{[1
]}

Zhu, Yongxing ^{[2
]}

Zhang, Liang ^{[1
]}

Zhang, Wei ^{[1
]}

Chen, Rumei ^{[1
]}

Xu, Miaoyun ^{[1
]}

Fan, Yunliu ^{[1
]}

Wang, Lei ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Biotechnol Res Inst, Beijing 100081, Peoples R China

[2] Ningxia Acad Agr & Forestry Sci, Agr Biotechnol Ctr, Ningxia 750002, Peoples R China

来源：

TRANSGENIC RESEARCH | 2013年 / 22卷 / 05期

基金：

国家高技术研究发展计划(863计划);

关键词：

Glycine Max; Maize; Vitamin E; Tocopherol; gamma-Tocopherol methyltransferase (gamma-TMT); alpha-Tocopherol; GmTMT2a; VITAMIN-E CONTENT; GAMMA-TMT GENE; HOMOGENTISATE PHYTYLTRANSFERASE; PERILLA-FRUTESCENS; PLANTS; METHYLTRANSFERASE; TRANSFORMATION; BIOSYNTHESIS; MUTANT;

D O I：

10.1007/s11248-013-9713-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tocochromanol, or vitamin E, plays a crucial role in human and animal nutrition and is synthesized only by photosynthetic organisms. gamma-Tocopherol methyltransferase (gamma-TMT), one of the key enzymes in the tocopherol biosynthetic pathway in plants, converts gamma, delta-tocopherols into alpha-, beta-tocopherols. Tocopherol content was investigated in 15 soybean cultivars and GmTMT2 was isolated from five varieties based on tocopherol content. GmTMT2a was expressed in E. coli and the purified protein effectively converted gamma-tocopherol into alpha-tocopherol in vitro. Overexpression of GmTMT2a enhanced alpha-tocopherol content 4-6-fold in transgenic Arabidopsis, and alpha-tocopherol content increased 3-4.5-fold in transgenic maize seed, which correlated with the accumulation of GmTMT2a. Transgenic corn that is alpha-tocopherol-rich may be beneficial for animal health and growth.

引用

页码：1021 / 1028

页数：8

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