Phenotype-Independent Effects of Retroviral Transduction in Human Dental Pulp Stem Cells

被引：7

作者：

Egbuniwe, Obi ^{[1
]}

Grant, Andrew D. ^{[2
]}

Renton, Tara ^{[3
]}

Di Silvio, Lucy ^{[1
]}

机构：

[1] Guys Hosp, Kings Coll London, London SE1 9RT, England

[2] Kings Coll London, Wolfson Ctr Age Related Dis, London SE1 1UL, England

[3] Kings Coll London, Inst Dent, London SE5 9RS, England

来源：

MACROMOLECULAR BIOSCIENCE | 2013年 / 13卷 / 07期

基金：

英国生物技术与生命科学研究理事会;

关键词：

dental pulp; immortalization; matrix proteins; odontoblasts; stem cells; TIME QUANTITATIVE PCR; TELOMERASE ACTIVITY; EXTRACELLULAR-MATRIX; GENE-EXPRESSION; LIFE-SPAN; OSTEOGENIC DIFFERENTIATION; MOLECULAR-STRUCTURE; NUCLEIC-ACIDS; MESSENGER-RNA; IN-VITRO;

D O I：

10.1002/mabi.201300020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

An immortalized human dental pulp stem cell (DPSC) line of an odontoblastic phenotype is established to circumvent the normal programmed senescence and to maintain the cell line's usefulness as a tool for further study of cellular activity. DPSCs are isolated from human dental pulp tissues and transfected using hTERT. The influence of this process on the DPSC phenotype and the mRNA expression of oncogenes involved in cellular senescence is investigated. The results reveal an absence of altered DPSC morphology and phenotype following the exogenous introduction of the hTERT gene, which is coupled with a significant reduction in p16 mRNA expression. This provides insight into how to circumvent in vitro dental pulp stem cell death following the exogenous introduction of hTERT.

引用

页码：851 / 859

页数：9

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