Effect of intestinal bacteria on formation of azoxymethane-induced aberrant crypt foci in the rat colon

被引：44

作者：

Arimochi, H ^{[1
]}

Kinouchi, T ^{[1
]}

Kataoka, K ^{[1
]}

Kuwahara, T ^{[1
]}

Ohnishi, Y ^{[1
]}

机构：

[1] UNIV TOKUSHIMA,SCH MED,DEPT BACTERIOL,TOKUSHIMA 770,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 238卷 / 03期

关键词：

D O I：

10.1006/bbrc.1997.7384

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of intestinal bacteria on formation of azoxymethane (AOM)-induced aberrant crypt foci (ACF) and DNA adducts in the rat colon was investigated. Male Sprague-Dawley rats were administered cultures of Lactobacillus acidophilus, Bifidobacterium adolescentis, Bacteroides fragilis, Escherichia coli and Clostridium perfringens for five weeks and given injections of AOM at 15 mg/kg body weight at the first and second weeks. The number of ACF five weeks after the start of the experiment was decreased in the rats treated with the cultures or culture supernatants of L. acidophilus and C. perfringens. The half-life of O-6-methylguanine (O-6-meG;) in the L. acidophilus group was shorter than that in the GAM broth group. The half-life of 7-methylguanine did not differ among the groups. These results suggest that the metabolite(s) of L. acidophilus and C. perfringens inhibit(s) the ACF formation in rats treated with AOM and that the inhibitory effect of L. acidophilus is due to the enhanced removal of O-6-meG from the colon mucosal DNA. (C) 1997 Academic Press.

引用

页码：753 / 757

页数：5

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