Characterization of the maize xylem sap proteome

被引:89
作者
Alvarez, S [1 ]
Goodger, JQD [1 ]
Marsh, EL [1 ]
Chen, SX [1 ]
Asirvatham, VS [1 ]
Schachtman, DP [1 ]
机构
[1] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA
关键词
xylem sap; proteome; isoform; glycoprotein; antifungal activity; maize; corn; plants;
D O I
10.1021/pr050471q
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The xylem in plants has mainly been described as a conduit for water and minerals, but emerging evidence also indicates that the xylem contains protein. To study the proteins in xylem sap, we characterized the identity and composition of the maize xylem sap proteome. The composition of the xylem sap proteome in maize revealed proteins related to different phases of xylem differentiation including cell wall metabolism, secondary cell wall synthesis, and programmed cell death. Many proteins were found to be present as multiple isoforms and some of these isoforms are glycosylated. Proteins involved in defense mechanisms were also present in xylem sap and the sap proteins were shown to have antifungal activity in bioassays.
引用
收藏
页码:963 / 972
页数:10
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