Molecular Architecture and Assembly Principles of Vibrio cholerae Biofilms

被引：297

作者：

Berk, Veysel ^{[1
]}

Fong, Jiunn C. N. ^{[2
]}

Dempsey, Graham T. ^{[3
]}

Develioglu, Omer N. ^{[6
]}

Zhuang, Xiaowei ^{[3
,4
,5
]}

Liphardt, Jan ^{[1
,7
]}

Yildiz, Fitnat H. ^{[2
]}

Chu, Steven ^{[8
]}

机构：

[1] Univ Calif Berkeley, Dept Phys, Berkeley, CA 94720 USA

[2] Univ Calif Santa Cruz, Dept Microbiol & Environm Toxicol, Santa Cruz, CA 95064 USA

[3] Harvard Univ, Biophys Program, Cambridge, MA 02138 USA

[4] Harvard Univ, Dept Phys, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

[5] Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA

[6] Taksim Training & Res Hosp, Dept Otorhinolaryngol, Istanbul, Turkey

[7] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

[8] US DOE, Washington, DC 20585 USA

来源：

SCIENCE | 2012年 / 337卷 / 6091期

关键词：

OPTICAL RECONSTRUCTION MICROSCOPY; RUGOSE COLONY MORPHOLOGY; BACTERIAL BIOFILMS; ANTIBIOTIC-RESISTANCE; PATHOGENESIS; DISPERSAL; MATRIX;

D O I：

10.1126/science.1222981

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In their natural environment, microbes organize into communities held together by an extracellular matrix composed of polysaccharides and proteins. We developed an in vivo labeling strategy to allow the extracellular matrix of developing biofilms to be visualized with conventional and superresolution light microscopy. Vibrio cholerae biofilms displayed three distinct levels of spatial organization: cells, clusters of cells, and collections of clusters. Multiresolution imaging of living V. cholerae biofilms revealed the complementary architectural roles of the four essential matrix constituents: RbmA provided cell-cell adhesion; Bap1 allowed the developing biofilm to adhere to surfaces; and heterogeneous mixtures of Vibrio polysaccharide, RbmC, and Bap1 formed dynamic, flexible, and ordered envelopes that encased the cell clusters.

引用

页码：236 / 239

页数：5

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