Isolation and structure determination of novamethymycin, a new bioactive metabolite of the methymycin biosynthetic pathway in Streptomyces venezuelae

被引：41

作者：

Zhang, QB

Sherman, DH ^{[1
]}

机构：

[1] Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Inst Biotechnol, Minneapolis, MN 55455 USA

来源：

JOURNAL OF NATURAL PRODUCTS | 2001年 / 64卷 / 11期

关键词：

D O I：

10.1021/np010146r

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Novamethymycin (9), a novel macrolide antibiotic, was isolated from Streptomyces venezuelae, the producer of methymycin (4) and neomethymycin (5). Spectroscopic analysis of 9 indicated that it is highly related to 4 and 5 but contains hydroxy groups at both C-10 and C-12 on the macrolactone ring. Bioconversion studies using the PikC cytochrome P450 hydroxylase demonstrated that 4 is converted to 9, further broadening the remarkable substrate flexibility of this enzyme.

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页码：1447 / 1450

页数：4

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