Purification and functional properties of Rab11-FIP3

被引：18

作者：

Horgan, CP ^{[1
]}

Zurawski, TH ^{[1
]}

McCaffrey, MW ^{[1
]}

机构：

[1] Natl Univ Ireland Univ Coll Cork, Dept Biochem, Mol Cell Biol Lab, Biosci Inst, Cork, Ireland

来源：

GTPASES REGULATING MEMBRANE TARGETING AND FUSION | 2005年 / 403卷

基金：

爱尔兰科学基金会;

关键词：

D O I：

10.1016/S0076-6879(05)03044-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Rab family of small GTPases are key regulators of membrane trafficking in eukaryotic cells. Rab11, one member of this family, plays a role in regulating various cellular functions such as plasma membrane recycling, phagocytosis, and cytokinesis. A family of Rab11-binding proteins has been identified and termed the Rab11 family interacting proteins or Rab11-FIPs. Rab11-FIP3, a member of this Rab11-binding protein family, in addition to interacting with Rab11, is also capable of interaction with members of the ADP-Ribosylation Factor (ARF) GTPase family. Here we describe the purification of Rab11-FIP3 and report its biological properties in eukaryotic cells as visualized by immunofluorescence microscopy.

引用

页码：499 / +

页数：15

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