Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter

被引:483
作者
Wu, Xiaolan [1 ]
Shiroto, Yoko [1 ]
Kishitani, Sachie [2 ]
Ito, Yukihiro [1 ]
Toriyama, Kinya [1 ]
机构
[1] Tohoku Univ, Grad Sch Agr Sci, Lab Environm Biotechnol, Aoba Ku, Sendai, Miyagi 9818555, Japan
[2] Tohoku Univ, Grad Sch Agr Sci, Lab Plant Breeding & Genet, Aoba Ku, Sendai, Miyagi 9818555, Japan
关键词
Drought tolerance; Heat tolerance; Transgenic rice; WRKY; WRKY TRANSCRIPTION FACTOR; RESPONSIVE GENE-EXPRESSION; LENGTH CDNA MICROARRAY; PLANT DEFENSE RESPONSE; DNA-BINDING PROTEINS; SALT TOLERANCE; BETA-GLUCURONIDASE; FREEZING TOLERANCE; TOBACCO PLANTS; SYNTHASE GENE;
D O I
10.1007/s00299-008-0614-x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
An OsWRKY11 gene, which encodes a transcription factor with the WRKY domain, was identified as one of the genes that was induced by both heat shock and drought stresses in seedlings of rice (Oryza sativa L.). To determine if overexpression of OsWRKY11 confers heat and drought tolerance, OsWRKY11 cDNA was fused to the promoter of HSP101 of rice and introduced into a rice cultivar Sasanishiki. Overexpression of OsWRKY11 was induced by heat treatment. After heat pretreatment, the transgenic lines showed significant heat and drought tolerance, as indicated by the slower leaf-wilting and less-impaired survival rate of green parts of plants. They also showed significant desiccation tolerance, as indicated by the slower water loss in detached leaves. Our results indicate that the OsWRKY11 gene plays a role in heat and drought stress response and tolerance, and might be useful for improvement of stress tolerance.
引用
收藏
页码:21 / 30
页数:10
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