The P2Y(1) receptor is an ADP receptor antagonized by ATP and expressed in platelets and megakaryoblastic cells

被引：254

作者：

Leon, C ^{[1
]}

Hechler, B ^{[1
]}

Vial, C ^{[1
]}

Leray, C ^{[1
]}

Cazenave, JP ^{[1
]}

Gachet, C ^{[1
]}

机构：

[1] ETSS, INSERM, U311, F-67065 STRASBOURG, FRANCE

来源：

FEBS LETTERS | 1997年 / 403卷 / 01期

关键词：

P2Y(1) purinoceptor; ADP receptor; human platelet; megakaryoblastic cell; heterologous expression;

D O I：

10.1016/S0014-5793(97)00022-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human P2Y(1) purinoceptor has been expressed in Jurkat cells and the effects of HPLC purified nucleotides on calcium movements were measured. The most potent agonist was 2-methylthio-ADP followed by ADP, ATP, Sp-ATP alpha S and B,gamma-methylene-ATP were competitive antagonists, Suramin and PPADS inhibited the effects of ADP, This pharmacological profile is tbe same as that of the so-called P2T purinoceptor responsible for platelet aggregation, which has not yet been identified, Using PCR we found the P2Y(1) receptor to be present in blood platelets and megakaryoblastic cell lines. These data suggest that the P2Y(1) receptor may be the elusive P2T receptor. (C) 1997 Federation of European Biochemical Societies.

引用

页码：26 / 30

页数：5

共 32 条

[1] Cloning and chromosomal localization of the human P2Y1 purinoceptor
Ayyanathan, K
Webbs, TE
Sandhu, AK
Athwal, RS
Barnard, EA
Kunapuli, SP
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 218 (03) : 783 - 788
[2] Barnard EA, 1996, CIBA F SYMP, V198, P166
[3] Burnstock G, 1996, DRUG DEVELOP RES, V38, P67, DOI 10.1002/(SICI)1098-2299(199605)38:1<67::AID-DDR9>3.0.CO
[4] 2-J
[5] A DUAL FUNCTION FOR ADENOSINE 5'-TRIPHOSPHATE IN THE REGULATION OF VASCULAR TONE - EXCITATORY COTRANSMITTER WITH NORADRENALINE FROM PERIVASCULAR NERVES AND LOCALLY RELEASED INHIBITORY INTRAVASCULAR AGENT
BURNSTOCK, G
KENNEDY, C
[J]. CIRCULATION RESEARCH, 1986, 58 (03) : 319 - 330
[6] Burnstock G., 1978, CELL MEMBRAIN RECEPT, P107, DOI DOI 10.1016/0014-5793(79)81367-8
[7] ATP, A PARTIAL AGONIST OF ATYPICAL P-2Y PURINOCEPTORS IN RAT-BRAIN MICROVASCULAR ENDOTHELIAL-CELLS
FEOLDE, E
VIGNE, P
BREITTMAYER, JP
FRELIN, C
[J]. BRITISH JOURNAL OF PHARMACOLOGY, 1995, 115 (07) : 1199 - 1203
[8] FILTZ TM, 1994, MOL PHARMACOL, V46, P8
[9] GACHET C, 1996, IN PRESS PLATELETS
[10] EFFECTS OF ANALOGS OF ADENINE-NUCLEOTIDES ON INCREASES IN INTRACELLULAR CALCIUM MEDIATED BY P2T-PURINOCEPTORS ON HUMAN BLOOD-PLATELETS
HALL, DA
HOURANI, SMO
[J]. BRITISH JOURNAL OF PHARMACOLOGY, 1993, 108 (03) : 728 - 733

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