Development of rainbow trout microsatellite markers from repeat enriched libraries

被引:63
作者
Rexroad, CE
Coleman, RL
Gustafson, AL
Hershberger, WK
Killefer, J
机构
[1] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Leetown, WV USA
[2] W Virginia Univ, Div Anim & Vet Sci, Morgantown, WV 26506 USA
关键词
microsatellite; rainbow trout; linkage mapping; enriched library;
D O I
10.1007/s10126-001-0058-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The efficiency of developing polymorphic microsatellite markers from 2 repeat enriched libraries was evaluated. Thirty-six polymorphic microsatellite markers were developed for rainbow trout, 27 of which were informative in a mapping family. The ability of each marker to amplify genomic DNA from other salmonids was also observed.
引用
收藏
页码:12 / 16
页数:5
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