1-DE MS and 2-D LC-MS analysis of the mouse bronchoalveolar lavage proteome

被引：36

作者：

Guo, YR

Ma, SF

Grigoryev, D

Van Eyk, J

Garcia, JGN

机构：

[1] Univ Chicago, Med Ctr, Chicago, IL 60637 USA

[2] Johns Hopkins Univ, Div Pulm & Crit Care Med, Sch Med, Baltimore, MD USA

[3] Johns Hopkins Univ, Div Cardiol, Sch Med, Baltimore, MD USA

来源：

PROTEOMICS | 2005年 / 5卷 / 17期

关键词：

1-DE; bronchoalveolar lavage; LC-MS/MS; mouse; proteome analysis;

D O I：

10.1002/pmic.200500052

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bronchoalveolar lavage fluid (BALF) is a complex mixture of proteins, which represents a unique clinically useful sampling of the lower respiratory tract. Many proteomic technologies can be used to characterize complex biological mixtures; however, it is not yet clear which technology(s) provide more information regarding the number of proteins identified and sequence coverage. In this study, we initially compared two common proteomic approaches, 2-D LC microESI MS/MS and I-DE followed by gel slice digestion, peptide extraction and peptide identification by MS in characterization of the mouse BALF proteome; secondly, we identified 297 unique proteins from the mouse BALF proteome, greatly expanded the BALF proteome by about threefold regardless of species.

引用

收藏

页码：4608 / 4624

页数：17

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