Vaccinia virus proteome: Identification of proteins in vaccinia virus intracellular mature virion particles

被引:211
作者
Chung, CS
Chen, CH
Ho, MY
Huang, CY
Liao, CL [1 ]
Chang, W
机构
[1] Acad Sinica, Inst Mol Biol, Taipei 115, Taiwan
[2] Acad Sinica, Genom Res Ctr, Taipei 115, Taiwan
关键词
D O I
10.1128/JVI.80.5.2127-2140.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Vaccinia virus is a large enveloped poxvirus with more than 200 genes in its genome. Although many poxvirus genomes have been sequenced, knowledge of the host and viral protein components of the virions remains incomplete. In this study, we used gel-free liquid chromatography and tandem mass spectroscopy to identify the viral and host proteins in purified vaccinia intracellular mature virions (IMV). Analysis of the proteins in the IW showed that it contains 75 viral proteins, including structural proteins, enzymes, transcription factors, and predicted viral proteins not known to be expressed or present in the IW. We also determined the relative abundances of the individual protein components in the IMV. Finally, 23 IMV-associated host proteins were also identified. This study provides the first comprehensive structural analysis of the infectious vaccinia virus IW.
引用
收藏
页码:2127 / 2140
页数:14
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