Anatomical and biochemical events during in vitro rooting of microcuttings from juvenile and mature phases of chestnut

被引:85
作者
Ballester, A
San-José, MC
Vidal, N
Fernández-Lorenzo, JL
Vietez, AM
机构
[1] CSIC, Inst Invest Agrobiol Galicia, Santiago De Compostela 15080, Spain
[2] Escuela Politecn Super, Dept Prod Vegetalgoy, Lugo 27002, Spain
关键词
adventitious rooting; anatomy; auxins; Castanea sativa Mill; chestnut; juvenile phase; mature phase; polyamines; tissue culture;
D O I
10.1006/anbo.1999.0865
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A comparative study of the in vitro rooting process of chestnut (Castanea sativa) shoots of the same genotype exhibiting juvenile (easy-to-root) and mature (difficult-to-root) characteristics is described. The two culture lines originated from shoots collected from the base (juvenile) and crown (mature:) of an 80-year-old tree. Anatomically, juvenile and mature shoots had a similar stem structure at the time of excision, the main difference being that secondary phloem and xylem were more developed in mature than in juvenile shoots. A substantial reactivation of cell division was observed in both shoot lines 48 h after the root inductive treatment with indole-3-butyric acid. Meristemoids and root primordia developed only in juvenile shoots, beginning 3 d after the inductive treatment, and the first adventitious roots emerged 10 d after treatment. However, in mature shoots periclinal divisions of cambial cells occurred, especially on the phloem side, maintaining the normal orientation of the cambial derivatives. No meristemoids formed in this proliferating tissue. During the time course of the rooting process, more endogenous indole-3-acetic acid (IAA) was detected in mature than in juvenile shoots, indicating that the level of IAA is not the limiting factor accounting for the lack of rooting capacity in mature shoots. The levels of polyamines (putrescine, spermine and spermidine) were also higher in mature than in juvenile shoots. (C) 1999 Annals of Botany Company.
引用
收藏
页码:619 / 629
页数:11
相关论文
共 45 条
[1]   CYTOLOGICAL EVENTS INDUCED BY THE INHIBITION OF POLYAMINE BIOSYNTHESIS IN THIN CELL-LAYERS OF TOBACCO [J].
ALTAMURA, MM ;
CAPITANI, F ;
CERCHIA, R ;
FALASCA, G ;
BAGNI, N .
PROTOPLASMA, 1993, 175 (1-2) :9-16
[2]   Root histogenesis in herbaceous and woody explants cultured in vitro. A critical review [J].
Altamura, MM .
AGRONOMIE, 1996, 16 (10) :589-602
[3]   RELATIONSHIP BETWEEN INDOLE-3-ACETIC-ACID LEVELS IN APPLE (MALUS-PUMILA MILL) ROOTSTOCKS CULTURED INVITRO AND ADVENTITIOUS ROOT-FORMATION IN THE PRESENCE OF INDOLE-3-BUTYRIC ACID [J].
ALVAREZ, R ;
NISSEN, SJ ;
SUTTER, EG .
PLANT PHYSIOLOGY, 1989, 89 (02) :439-443
[4]  
BALKESLEY D, 1994, BIOL ADVENTITIOUS RO, P143
[5]  
BALLESTER A, 1996, AIR3CT942202 EUR COM, P42
[6]  
BARALDI R, 1995, J PLANT GROWTH REGUL, V14, P49, DOI 10.1007/BF00212646
[7]   ENDOGENOUS LEVELS OF PLANT HORMONES DURING THE COURSE OF ADVENTITIOUS ROOTING IN CUTTINGS OF SEQUOIADENDRON-GIGANTEUM (LINDL) INVITRO [J].
BERTHON, JY ;
MALDINEY, R ;
SOTTA, B ;
GASPAR, T ;
BOYER, N .
BIOCHEMIE UND PHYSIOLOGIE DER PFLANZEN, 1989, 184 (5-6) :405-412
[8]  
BIONDI S, 1990, PHYSIOL PLANTARUM, V78, P474, DOI 10.1111/j.1399-3054.1990.tb09066.x
[9]  
Collichio F, 1994, Oncology (Williston Park), V8, P45
[10]   POLYAMINE EVOLUTION IN BUDS AND SHOOTS OF DOUGLAS-FIR (PSEUDOTSUGA-MENZIESII) AFTER THE TRANSITION FROM VEGETATIVE DEVELOPMENT TO SEXUAL DEVELOPMENT [J].
DAOUDI, EH ;
BONNETMASIMBERT, M ;
MARTINTANGUY, J .
ANNALES DES SCIENCES FORESTIERES, 1991, 48 (04) :367-376