Coupling fluorescence correlation spectroscopy with microchip electrophoresis to determine the effective surface charge of water-soluble quantum dots

被引：32

作者：

Dong, CQ ^{[1
]}

Bi, R ^{[1
]}

Qian, HF ^{[1
]}

Li, L ^{[1
]}

Ren, JC ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China

来源：

SMALL | 2006年 / 2卷 / 04期

关键词：

electrophoresis; fluorescence spectroscopy; quantum dots; surface charges;

D O I：

10.1002/smll.200500456

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An innovative method to determine the surface charge of quantum dots (QD) by coupling fluorescence correlation spectroscopy (FCS) with microchip electrophoresis was investigated. It was shown that the surface charge of QDs was remarkably closely associated with the type of stabilizer on the QD surface, the buffer value, and other factors. It was suggested that the technique can be extended to measure various fluorescent compounds from small molecules to nanoparticles. It was found that the technique can be used to investigate donor-receptor interactions, bioconjugation, and drug steering in colloidal chemistry.

引用

页码：534 / 538

页数：5

共 29 条

[1] The use of nanocrystals in biological detection [J].

Alivisatos, P .

NATURE BIOTECHNOLOGY, 2004, 22 (01) :47-52

[2] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[3] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[4] Ultraviolet sealing and poly(dimethylacrylamide) modification for poly(dimethylsiloxane)/glass microchips [J].

Chen, L ;

Ren, JC ;

Bi, R ;

Chen, D .

ELECTROPHORESIS, 2004, 25 (06) :914-921

[5] Imaging the photoionization of individual CdSe/CdS core-shell nanocrystals on n- and p-type silicon substrates with thin oxides [J].

Cherniavskaya, O ;

Chen, LW ;

Brus, L .

JOURNAL OF PHYSICAL CHEMISTRY B, 2004, 108 (16) :4946-4961

[6] Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy [J].

Doose, S ;

Tsay, JM ;

Pinaud, F ;

Weiss, S .

ANALYTICAL CHEMISTRY, 2005, 77 (07) :2235-2242

[7] In vivo imaging of quantum dots encapsulated in phospholipid micelles [J].

Dubertret, B ;

Skourides, P ;

Norris, DJ ;

Noireaux, V ;

Brivanlou, AH ;

Libchaber, A .

SCIENCE, 2002, 298 (5599) :1759-1762

[8] FLUORESCENCE CORRELATION SPECTROSCOPY .1. CONCEPTUAL BASIS AND THEORY [J].

ELSON, EL ;

MAGDE, D .

BIOPOLYMERS, 1974, 13 (01) :1-27

[9] Avidin: A natural bridge for quantum dot-antibody conjugates [J].

Goldman, ER ;

Balighian, ED ;

Mattoussi, H ;

Kuno, MK ;

Mauro, JM ;

Tran, PT ;

Anderson, GP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (22) :6378-6382

[10] Biological and chemical applications of fluorescence correlation spectroscopy: A review [J].

Hess, ST ;

Huang, SH ;

Heikal, AA ;

Webb, WW .

BIOCHEMISTRY, 2002, 41 (03) :697-705

← 1 2 3 →