Optimization of the enzymatic synthesis of O-glycan core 2 structure by use of a genetic algorithm

被引：9

作者：

Hoh, C ^{[1
]}

Dudziak, G ^{[1
]}

Liese, A ^{[1
]}

机构：

[1] Forschungszentrum Julich, Inst Biotechnol 2, D-52425 Julich, Germany

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2002年 / 12卷 / 07期

关键词：

D O I：

10.1016/S0960-894X(02)00087-2

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The enzymatic synthesis of Gal-beta1,3[GlcNAc-beta1,6]-GalNAc-alpha1-OBn (core 2-Bn) using a multi-enzyme system consisting of a beta-galactosidase (EC 3.2.1.23) from bovine testes and a recombinant core 2 beta1,6-GlcNAc transferase (C2GnT, EC 2.4.1.102) was empirically optimized by the use of a genetic algorithm. After variation of seven relevant parameters and performance of 56 experiments, two local maxima regarding the selection criterias could be found after four generations of optimization. The selectivity of core 2-Bn formation showed values up to 90%. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1031 / 1034

页数：4

共 24 条

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