Steroid transformation by mutants of Mycobacterium sp with altered response to antibiotics

被引：15

作者：

Barthakur, S ^{[1
]}

Roy, MK ^{[1
]}

Bera, SK ^{[1
]}

Ghosh, AC ^{[1
]}

机构：

[1] REG RES LAB,DIV BIOCHEM,JORHAT 785006,ASSAM,INDIA

来源：

JOURNAL OF BASIC MICROBIOLOGY | 1996年 / 36卷 / 06期

关键词：

ARTHROBACTER-OXYDANS; DEGRADATION; CLEAVAGE; STRAIN;

D O I：

10.1002/jobm.3620360602

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mycobacterium sp. NRRLB3683 which is capable to convert beta-sitosterol to 1,4-androstadiene-3,17-dione (ADD) was treated with methyl methane sulfonate and two strains with altered sensitivity to various antibiotics were obtained. One of the strain was steroid 1(2)-dehydrogenase negative and the other positive. Efficiency of utilization of sterols followed the order beta-sitosterol > cholesterol > soluble cholesterol. The steroid 1(2)-dehydrogenase negative strain was capable of producing 17KS (AD) from beta-sitosterol and converting AD to testosterone and ADD to AD suggesting the negative role of 1(2)dehydrogenase in sterol side chain cleavage and decrease in hydrogenase activity by mutation. But this enzyme can perform the reverse reaction under aerobic condition.

引用

页码：383 / 387

页数：5

共 15 条

[1]

CHOI SK, 1988, J APPL MICROBIOL BIO, V16, P187

[2] METABOLIC BLOCKS IN THE DEGRADATION OF BETA-SITOSTEROL BY A PLASMID-CURED STRAIN OF ARTHROBACTER-OXYDANS [J].