Gene amplification in amphotericin B-resistant Leishmania tarentolae

被引：33

作者：

Singh, AK

Papadopoulou, B

Ouellette, M

机构：

[1] CHU Laval, Ctr Rech, Ctr Rech Infect, St Foy, PQ G1V 4G2, Canada

[2] Univ Laval, Fac Med, Dept Microbiol, Quebec City, PQ G1V 4G2, Canada

来源：

EXPERIMENTAL PARASITOLOGY | 2001年 / 99卷 / 03期

基金：

加拿大健康研究院;

关键词：

amphotericin B; Leishmania drug resistance; gene amplification;

D O I：

10.1006/expr.2001.4663

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Singh, A. K., Papadopoulou, B., and Ouellette, M. 2001. Gene amplification in amphotericin B-resistant Leishmania tarentolae. Experimental Parasitology 99, 141-147. Two Leishmania tarentolae cells were selected step by step for resistance to the polyene antibiotic amphotericin B, a second-line drug against the parasite Leishmania. One of the mutants was cross-resistant to ketoconazole. DNA amplification was observed in both mutants. The amplicons were extrachromosomal circles and were derived from different chromosomes. In one mutant the circle was unusually stable as it remained within the cell despite numerous passages in the absence of the drug. A circumstantial link between the copy number of amplicons and the resistance levels was established. Gene transfection experiments indicated that the link between the locus amplified and the resistance levels was not straightforward and possibly several mutations act together to lead to amphotericin B resistance. (C) 2001 Elsevier Science (USA).

引用

页码：141 / 147

页数：7

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