Temporal variability and effect of environmental variables on airborne bacterial communities in an urban area of Northern Italy

被引:173
作者
Bertolini, Valentina [1 ]
Gandolfi, Isabella [1 ]
Ambrosini, Roberto [2 ]
Bestetti, Giuseppina [1 ]
Innocente, Elena [3 ]
Rampazzo, Giancarlo [3 ]
Franzetti, Andrea [1 ]
机构
[1] Univ Milano Bicocca, Dept Environm Sci DISAT, POLARIS Res Ctr, I-20126 Milan, Italy
[2] Univ Milano Bicocca, Dept Biotechnol & Biosci, I-20126 Milan, Italy
[3] Univ Ca Foscari, Dept Environm Sci Informat & Stat, I-30123 Venice, Italy
关键词
16S rRNA; Airborne bacteria; Community structure; Illumina sequencing; Multivariate regression trees; 16S RIBOSOMAL-RNA; REAL-TIME PCR; PARTICULATE MATTER; AIR; DIVERSITY; MICROORGANISMS; IDENTIFICATION; PATHOGENS;
D O I
10.1007/s00253-012-4450-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Despite airborne microorganisms representing a relevant fraction of atmospheric suspended particles, only a small amount of information is currently available on their abundance and diversity and very few studies have investigated the environmental factors influencing the structure of airborne bacterial communities. In this work, we used quantitative PCR and Illumina technology to provide a thorough description of airborne bacterial communities in the urban area of Milan (Italy). Forty samples were collected in 10-day sampling sessions, with one session per season. The mean bacterial abundance was about 10(4) ribosomal operons per m(3) of air and was lower in winter than in the other seasons. Communities were dominated by Actinobacteridae, Clostridiales, Sphingobacteriales and few proteobacterial orders (Burkholderiales, Rhizobiales, Sphingomonadales and Pseudomonadales). Chloroplasts were abundant in all samples. A higher abundance of Actinobacteridae, which are typical soil-inhabiting bacteria, and a lower abundance of chloroplasts in samples collected on cold days were observed. The variation in community composition observed within seasons was comparable to that observed between seasons, thus suggesting that airborne bacterial communities show large temporal variability, even between consecutive days. The structure of airborne bacterial communities therefore suggests that soil and plants are the sources which contribute most to the airborne communities of Milan atmosphere, but the structure of the bacterial community seems to depend mainly on the source of bacteria that predominates in a given period of time.
引用
收藏
页码:6561 / 6570
页数:10
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