Cytotoxicity assessment of graphene-based nanomaterials on human dental follicle stem cells

被引：62

作者：

Olteanu, Diana ^{[1
]}

Filip, Adriana ^{[1
]}

Socaci, Crina ^{[2
]}

Biris, Alexandru Radu ^{[2
]}

Filip, Xenia ^{[2
]}

Coros, Maria ^{[2
]}

Rosu, Marcela Corina ^{[2
]}

Pogacean, Florina ^{[2
]}

Alb, Camelia ^{[3
]}

Baldea, Ioana ^{[1
]}

Bolfa, Pompei ^{[4
,5
]}

Pruneanu, Stela ^{[2
]}

机构：

[1] Iuliu Hatieganu Univ Med & Pharm, Dept Physiol, Clinicilor 1-3, Cluj Napoca, Romania

[2] Natl Inst Res & Dev Isotop & Mol Technol, Cluj Napoca 400293, Romania

[3] Iuliu Hatieganu Univ Med & Pharm, Fac Dent Med, Dept Propedeut & Dent Mat, Cluj Napoca, Romania

[4] Univ Agr Sci & Vet Med, Dept Pathol, Cluj Napoca 400372, Romania

[5] Ross Univ, Sch Vet Med Basseterre, Dept Biomed Sci, Basseterre, St Kitts & Nevi

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2015年 / 136卷

关键词：

Graphene based nanomaterials; Human dental follicle stem cells; Cytotoxicity assessment; CARBON NANOTUBES; COMPOSITE FILMS; OXIDE; REDUCTION; PROLIFERATION; MECHANISMS; TOXICITY; SENSORS; SIZE;

D O I：

10.1016/j.colsurfb.2015.10.023

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

Grapheme-oxide (GO) and its most encountered derivatives, thermally reduced graphene oxide (TRGO) and nitrogen-doped graphene (N-Gr), were synthesized and structurally characterized by spectroscopic techniques, like Raman and C-13 MAS solid state NMR. Several biological effects (cytotoxicity, oxidative stress induction, and cellular and mithocondrial membrane alterations) induced by such graphene-based materials on human dental follicle stem cells were investigated. Grapheme oxide shows the lowest cytotoxic effect, followed by the nitrogen-doped graphene, while thermally reduced graphene oxide exhibits high cytotoxic effects. Graphene oxide induces oxidative stress without causing cell membrane damage. Nitrogen-doped graphene shows a slight antioxidant activity; however, at high doses (20 and 40 mu g/ml) it causes membrane damage. Both grapheme oxide and nitrogen-doped graphene seem to be valuable candidates for usage in dental nanocomposites. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：791 / 798

页数：8

共 46 条

[1]

Protein Degradation and RNA Efflux of Viruses Photocatalyzed by Graphene-Tungsten Oxide Composite Under Visible Light Irradiation [J].

Akhavan, O. ;

Choobtashani, M. ;

Ghaderi, E. .

JOURNAL OF PHYSICAL CHEMISTRY C, 2012, 116 (17) :9653-9659

[2]

Toxicity of Graphene and Graphene Oxide Nanowalls Against Bacteria [J].

Akhavan, Omid ;

Ghaderi, Elham .

ACS NANO, 2010, 4 (10) :5731-5736

[3]

Synthesis and solid-state NMR structural characterization of 13C-labeled graphite oxide [J].

Cai, Weiwei ;

Piner, Richard D. ;

Stadermann, Frank J. ;

Park, Sungjin ;

Shaibat, Medhat A. ;

Ishii, Yoshitaka ;

Yang, Dongxing ;

Velamakanni, Aruna ;

An, Sung Jin ;

Stoller, Meryl ;

An, Jinho ;

Chen, Dongmin ;

Ruoff, Rodney S. .

SCIENCE, 2008, 321 (5897) :1815-1817

[4]

General equation for the determination of the crystallite size La of nanographite by Raman spectroscopy [J].

Cançado, LG ;

Takai, K ;

Enoki, T ;

Endo, M ;

Kim, YA ;

Mizusaki, H ;

Jorio, A ;

Coelho, LN ;

Magalhaes-Paniago, R ;

Pimenta, MA .

APPLIED PHYSICS LETTERS, 2006, 88 (16)

[5]

In vitro toxicity evaluation of graphene oxide on A549 cells [J].

Chang, Yanli ;

Yang, Sheng-Tao ;

Liu, Jia-Hui ;

Dong, Erya ;

Wang, Yanwen ;

Cao, Aoneng ;

Liu, Yuanfang ;

Wang, Haifang .

TOXICOLOGY LETTERS, 2011, 200 (03) :201-210

[6]

CONTI M, 1991, CLIN CHEM, V37, P1273

[7]

Perspectives on Carbon Nanotubes and Graphene Raman Spectroscopy [J].

Dresselhaus, Mildred S. ;

Jorio, Ado ;

Hofmann, Mario ;

Dresselhaus, Gene ;

Saito, Riichiro .

NANO LETTERS, 2010, 10 (03) :751-758

[8]

Gao W, 2009, NAT CHEM, V1, P403, DOI [10.1038/NCHEM.281, 10.1038/nchem.281]

[9]

PREPARATION OF GRAPHITIC OXIDE [J].

HUMMERS, WS ;

OFFEMAN, RE .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1958, 80 (06) :1339-1339

[10]

Processing of nanographene platelets (NGPs) and NGP nanocomposites: a review [J].

Jang, B. Z. ;

Zhamu, A. .

JOURNAL OF MATERIALS SCIENCE, 2008, 43 (15) :5092-5101

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