Adenovirus-mediated gene transfer of adiponectin reduces the severity of collagen-induced arthritis in mice

被引：41

作者：

Ebina, Kosuke ^{[1
,2
]}

Shima, Kazuya ^{[1
,2
]}

Matsuda, Morihiro ^{[3
,4
]}

Fukuhara, Atsunori

Maeda, Kazuhisa

Kihara, Shinji

Hashimoto, Jun ^{[1
,2
]}

Ochi, Takahiro ^{[5
]}

Banda, Nirmal K. ^{[6
,7
]}

Yoshikawa, Hideki ^{[1
,2
]}

Shimomura, Iichiro ^{[1
,2
]}

机构：

[1] Osaka Univ, Grad Sch Med, Dept Orthopaed, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Med, Dept Metab Med, Suita, Osaka 5650871, Japan

[3] Natl Hosp Org Kure Med Ctr, Inst Clin Res, Div Anal Pathophysiol, Hiroshima 7370023, Japan

[4] Natl Hosp Org Kure Med Ctr, Dept Internal Med, Hiroshima 7370023, Japan

[5] Osaka Police Hosp, Osaka 5430035, Japan

[6] Univ Colorado Denver, Div Rheumatol B115, Aurora, CO 80045 USA

[7] Hlth Sci Ctr, Aurora, CO 80045 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 378卷 / 02期

关键词：

Adiponectin; Collagen-induced arthritis; Complement; Disease severity; Inflammation; Mice; Rheumatoid arthritis; RHEUMATOID-ARTHRITIS; T-CELLS; COMPLEMENT; INDUCTION; PATHWAY; RECEPTOR; PROTEIN; MACROPHAGES; INHIBITION; ACTIVATION;

D O I：

10.1016/j.bbrc.2008.11.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Adiponectin (APN) is a hormone released by adipose tissue with anti-inflammatory properties. The purpose Of this study was to examine the therapeutic effects of systemic delivery of APN in murine arthritis model. Collagen-induced arthritis (CIA) was induced in male DBA1/J mice, and adenoviral vectors encoding human APN (Ad-APN) or beta-galactosidase (Ad-beta-gal) as control were injected either before or during arthritis progression. Systemic APN delivery at both time points significantly decreased clinical disease activity scores of CIA. In addition, APN treatment before arthritis progression significantly decreased histological scores of inflammation and cartilage damage, bone erosion, and mRNA levels of pro-inflammatory cytokines in the joints, without altering set-Urn anti-collagen antibodies levels. Immunohistochemical staining showed significant inhibition of complement C1q and C3 deposition in the joints of Ad-APN infected CIA mice. These results provide novel evidence that systemic APN delivery prevents inflammation and joint destruction ill murine arthritis model. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：186 / 191

页数：6

共 27 条

[1]

Pathogenic complement activation in collagen antibody-induced arthritis in mice requires amplification by the alternative pathway [J].