Xenon induces transcription of ADNP in neonatal rat brain

被引：26

作者：

Cattano, Davide ^{[2
]}

Valleggi, Simona ^{[1
]}

Ma, Daqing ^{[4
]}

Kastsiuchenka, Olga ^{[3
]}

Abramo, Antonio

Sun, Pamela ^{[4
]}

Cavazzana, Andrea O. ^{[1
]}

Natale, Gianfranco ^{[3
]}

Maze, Mervyn ^{[4
]}

Giunta, Francesco

机构：

[1] AUOP S Chiara Hosp, Mol Pathol Sect, Dept Oncol, Pisa, Italy

[2] Washington Univ, Sch Med, Dept Anesthesiol, St Louis, MO 63110 USA

[3] Univ Pisa, Sch Med, Dept Human Morphol & Appl Biol, I-56126 Pisa, Italy

[4] Univ London Imperial Coll Sci Technol & Med, Dept Anaesthet Pain Med & Intens Care, London, England

来源：

NEUROSCIENCE LETTERS | 2008年 / 440卷 / 03期

基金：

英国医学研究理事会;

关键词：

suppression subtractive hybridization; gene transcription; neuroprotection; anesthesia induced preconditioning;

D O I：

10.1016/j.neulet.2008.05.086

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Xenon and other inhalational agents induce cell and organ protection through different and only partially elucidated molecular mechanisms. Anesthesia induced or pharmacologic preconditioning is a recognized mechanism of cell protection. In this study we explored the gene transcription of activity-dependent neuroprotective protein (ADNP) in neonatal rat brain as consequence to xenon exposure, comparing the noble gas to nitrogen. Seven-day-old Sprague Dawley rats were exposed for 120 min to 75% xenon and 25% oxygen or control condition consisting of 75% nitrogen and 25% oxygen (Air). ADNP was found to be differentially expressed by SSH, validated by Relative Real-Time PCR (RT-PCR) and confirmed by western blot and immunohistochemistry. The differential expression of ADNP in the rat neonatal brain may account for the preconditioning and neuroprotective effects exerted by gas xenon. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：217 / 221

页数：5

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