A Tunable Ratiometric pH Sensor Based on Carbon Nanodots for the Quantitative Measurement of the Intracellular pH of Whole Cells

被引：420

作者：

Shi, Wen ^{[1
]}

Li, Xiaohua ^{[1
]}

Ma, Huimin ^{[1
]}

机构：

[1] Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 26期

关键词：

carbon nanodots; fluorescent probes; intracellular pH; ratiometric imaging; sensors; FLUORESCENT-PROBE; LIVING CELLS; OXIDATIVE STRESS; DRUG-DELIVERY; LIVE CELLS; NANOPARTICLES; DOTS; APOPTOSIS; ACTIVATION; NANOTUBES;

D O I：

10.1002/anie.201202533

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The whole picture: Carbon nanodots labeled with two fluorescent dyes have been developed as a tunable ratiometric pH sensor to measure intracellular pH. The nanosensor shows good biocompatibility and cellular dispersibility. Quantitative determinations on intact HeLa cells and pH fluctuations associated with oxidative stress were performed. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：6432 / 6435

页数：4

共 59 条

[1] Delivery and Subcellular Targeting of Dendrimer-Based Fluorescent pH Sensors in Living Cells [J].

Albertazzi, Lorenzo ;

Storti, Barbara ;

Marchetti, Laura ;

Beltram, Fabio .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (51) :18158-18167

[2]

[Anonymous], 2010, ANGEW CHEM INT EDIT, DOI [DOI 10.1002/ange.200906154, DOI 10.1002/ANGE.200906154]

[3]

[Anonymous], 2010, ANGEW CHEM-GER EDIT, DOI DOI 10.1002/ANGE.201000982

[4]

Baker S. N., 2010, ANGEW CHEM, V122, P6876, DOI [10.1002/ange.200906623, DOI 10.1002/ANGE.200906623]

[5] Luminescent Carbon Nanodots: Emergent Nanolights [J].

Baker, Sheila N. ;

Baker, Gary A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (38) :6726-6744

[6] Evaluating Nanoparticle Sensor Design for Intracellular pH Measurements [J].

Benjaminsen, Rikke V. ;

Sun, Honghao ;

Henriksen, Jonas R. ;

Christensen, Nynne M. ;

Almdal, Kristoffer ;

Andresen, Thomas L. .

ACS NANO, 2011, 5 (07) :5864-5873

[7] Development of a novel GFP-based ratiometric excitation and emission pH indicator for intracellular studies [J].

Bizzarri, R ;

Arcangeli, C ;

Arosio, D ;

Ricci, F ;

Faraci, P ;

Cardarelli, F ;

Beltram, F .

BIOPHYSICAL JOURNAL, 2006, 90 (09) :3300-3314

[8] FLUORESCENCE RATIO IMAGING MICROSCOPY - TEMPORAL AND SPATIAL MEASUREMENTS OF CYTOPLASMIC PH [J].

BRIGHT, GR ;

FISHER, GW ;

ROGOWSKA, J ;

TAYLOR, DL .

JOURNAL OF CELL BIOLOGY, 1987, 104 (04) :1019-1033

[9] Carbon dots for multiphoton bioimaging [J].

Cao, Li ;

Wang, Xin ;

Meziani, Mohammed J. ;

Lu, Fushen ;

Wang, Haifang ;

Luo, Pengju G. ;

Lin, Yi ;

Harruff, Barbara A. ;

Veca, L. Monica ;

Murray, Davoy ;

Xie, Su-Yuan ;

Sun, Ya-Ping .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (37) :11318-+

[10] Development of Ultrabright Semiconducting Polymer Dots for Ratiometric pH Sensing [J].

Chan, Yang-Hsiang ;

Wu, Changfeng ;

Ye, Fangmao ;

Jin, Yuhui ;

Smith, Polina B. ;

Chiu, Daniel T. .

ANALYTICAL CHEMISTRY, 2011, 83 (04) :1448-1455

← 1 2 3 4 5 6 →