Calcium release from InsP3-sensitive internal stores initiates action potential in Chara

被引：42

作者：

Biskup, B ^{[1
]}

Gradmann, D ^{[1
]}

Thiel, G ^{[1
]}

机构：

[1] Univ Gottingen, Biophys Abt, Albert von Haller Inst Pflanzenwissensch, D-37073 Gottingen, Germany

来源：

FEBS LETTERS | 1999年 / 453卷 / 1-2期

关键词：

Chara action potential; internal calcium store; excitatory reaction pathway; computer simulation; neomycin; U73122;

D O I：

10.1016/S0014-5793(99)00600-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neomycin and U73122 are known to suppress inositol 1,4,5-trisphosphate (InsP(3)) production by inhibition of phospholipase C. We studied the effects of these inhibitors on the excitatory currents, I-ex, in Chara corallina under voltage-clamp condition;. Computer simulations of the experimental effects by a minimum model for the excitatory reaction pathway allow the assignment of the inhibitory effects to one specific reaction step, i.e. the release of Ca2+ from InsP(3)-sensitive internal stores. In contrast, the inhibitory effect of La3+ on I-ex suggests inactivation of Cl- channels, Furthermore, ryanodine-sensitive Ca2+ stores seem to be irrelevant for electrical excitation in Chara. (C) 1999 Federation of European Biochemical Societies.

引用

页码：72 / 76

页数：5

共 37 条

[1]

BEILBY MJ, 1979, AUST J PLANT PHYSIOL, V6, P323

[2] CALCIUM AND PLANT ACTION-POTENTIALS [J].