Neutralization of a snake venom hemorrhagic metalloproteinase prevents coagulopathy after subcutaneous injection of Bothrops jararaca venom in rats

被引：45

作者：

Anai, K ^{[1
]}

Sugiki, M ^{[1
]}

Yoshida, E ^{[1
]}

Maruyama, M ^{[1
]}

机构：

[1] Miyazaki Med Coll, Dept Physiol, Miyazaki 8891692, Japan

来源：

TOXICON | 2002年 / 40卷 / 01期

关键词：

coagulopathy; snake venom; hemorrhagic metalloproteinase; Bothrops jararaca;

D O I：

10.1016/S0041-0101(01)00189-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Coagulopathy is one of the major complications following envenomations by crotalid and viperid snakes. The present study was undertaken to examine the effect of a hemorrhagic metalloproteinase in Borhrops jararaca venom, jararafibrase I (JF I), on the development of coagulopathy using rat snakebite model. Coagulation parameters were monitored after subcutaneous injection of B. jararaca crude venom, JF I-neutralizcd venom and purified JF I in rats. Crude venom induced unclottable blood and fibrinogen consumption, while JF I-neutralized venom and purified JF I did not induce coagulopathy. Plasma venom antigen level of rats given JF I-neutralized venom was lower than that of rats given crude venom. We conclude that venom hemorrhagic metalloproteinases play an important role in the development of coagulopathy through rapid spreading of venom coagulation components from the injected area into systemic circulation. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：63 / 68

页数：6

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