High-performance liquid chromatographic purification and capillary electrophoresis quantification of the chemokine stromal cell-derived factor-1

被引：3

作者：

Arcelloni, C

Aiuti, A

Cipponi, A

Paroni, R

机构：

[1] IRCCS, Dept Lab Med, Lab Separat Techniques, I-20132 Milan, Italy

[2] DIBIT, Telethon Inst Gene Therapy, I-20132 Milan, Italy

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 729卷 / 1-2期

关键词：

chemokines; SDF-1; alpha;

D O I：

10.1016/S0378-4347(99)00154-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC-chemokine stromal cell derived factor-1 (SDF-1 alpha) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatography. Yield and purity were assessed by capillary electrophoresis (CE) with reference to the human SDF-1 alpha from recombinant DNA technology. CE technique was useful to evaluate the purity of human SDF-1 alpha from chemical synthesis and to resolve murine and human SDF-1 alpha, differing by only one amino acid. Chemotactic activity of the murine SDF-1 alpha was tested on the basis of CE quantification. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：369 / 374

页数：6

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